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- 文献和实验
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- 规格:
20 µL/100 µL
| 规格: | 20 µL | 产品价格: | ¥600 |
|---|---|---|---|
| 规格: | 100 µL | 产品价格: | ¥1699 |
抗体种属:Human
抗体靶点:IL12B
抗体应用:ELISA
抗原货号:10052-H08H
抗原描述:Recombinant Human IL12B protein (Catalog#10052-H08H)
抗体宿主:Mouse
抗体Ig类型:Mouse IgG1
抗体纯化方法:Protein A
抗体制备:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL12B (rhIL12B; Catalog#10052-H08H; Met 1-Ser 328; NP_002178.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性:Human IL12B / P40
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验1, CALM2, CALM3, CAMK2B, CAMK4, CNR1, ITK, MAP2K7, NCK2, PAK1, PPIA, PPP3CB, PPP3CC, PPP3R1, TRPV6, VAV1, VAV2, VAV3. Ligands: CCL2, CSF2, CSF3, IFNA1, IFNB1, IFNG, IL1A, IL1B, IL2, IL3, IL4, IL6, IL8, IL10, IL12A, IL12B, IL13, IRF1, LTA, TGFB1, TNF
蛋白质印迹流程点击下载:蛋白质印迹实验方案溶液和试剂●裂解缓冲液这些缓冲液可在 4 ℃ 下保存数周,或者以分装形式在 -20 ℃ 下保存长达 1 年。Nonidet-P40 (NP40) 缓冲液150 mM NaCl1.0% NP40(可用 0.1% Triton X-100 替代)50 mM Tris-HCl pH 8.0蛋白酶抑制剂RIPA 缓冲液(放射免疫沉淀试验缓冲液)150 mM NaCl1.0% NP-40 或 0.1% Triton X-1000.5% 脱氧胆酸钠0.1% SDS
Buffer 0.32 M Sucrose 10mM Tris-HCl (pH 7.5) 5 mM MgCl2 1% v/v Triton X-100 2) PBND ( Buffer with Nonionic Detergents)* 50 mM KCl 10 mM Tris-HCl (pH 8.3) 2.5 mM MgCl2 0.1 mg/ml gelatin 0.45% (v/v) Nonidet P40 0.45% (v/v) Tween 20
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