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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant CD59 Protein
- 亚型:
IgG1
- 形态:
liquid
- 保存条件:
Shipped at 4℃. Upon delivery aliquot and store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
Non-conjugated
- 适应物种:
human,Other species are not tested.Please decide the specificity by homology
- 宿主:
mouse
- 应用范围:
ELISA, WB, IHC and other possible application
- 靶点:
CD59 glycoprotein,HRF-20,MAC-IP,MACIF,MIRL,CD59
- 抗体英文名:
Mouse Anti-CD59 monoclonal antibody
- 抗体名:
Mouse Anti-CD59 monoclonal antibody
- 规格:
100ug
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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