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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
+2 to +8°C
- 保质期:
36months
- 英文名:
Germall 115, reduced sodium
- 供应商:
上海汇谱仪器有限公司
- CAS号:
39236-46-9
Use Germall 115, reduced sodium as preservative in combination with other
biocides to obtain the best possible antimicrobial effect in tests that require a
low concentration of sodium.
Benefits
■ Take advantage of the strongly reduced concentration of sodium.
■ Combine Germall 115, reduced sodium with non-ionic, ionic detergents
or proteins.
■ Use Germall 115, reduced sodium in your preferred combination in liquid
reagents to obtain the most stable system.
CAS: 39236-46-9
Properties
Formula: C11H16N8O8 x H2O
Molecular weight: 406.31 D
Antimicrobial effect: Only moderate effect against fungi and yeast; should be
used in combination.
Possible combination: Can be combined with all biozides which do not seperate
formaldehyde, especially 5-bromo-5-nitro-1,3-dioxane (BND), 2-hydroxypyridine-
N-oxide (HPO) or N-methylisothiazolone (MIT).
Effect: Belongs to the ''formaldehyde-depot-substances''. Inactivating effect
due to reactions with -COOH, -NH2, -OH and -SH of all proteins.
Toxicity: Harmless at recommended concentrations; not carcinogenic or mutagenic
(Ames-test negative).
Recommended working concentration: 2.6 mg/ml
pH optimum: 4.5-8.5
Stability: At pH 6 and heating up to >+60°C this product decomposes with
separation of formaldehyde.
Specification
Appearance: White, crystalline powder
Solubility: Clear, colorless solution in water (c=10 mg/ml)
pH value (c=10 mg/ml, water): 2.8-3.2
Identity (NIR-spectrum): Corresponds to reference
Germall 115 (from N) : ≥94%
N (elementary analysis): ≥26%
Water (K. Fischer): ≤5%
HPTLC: Corresponds to reference
Heavy metals (as Pb): ≤20 ppm
Na (AES): ≤250 ppm
Reducing substances (as O): ≤0.5%
Oxidizing substances: ≤0.03%
Stability: At +2 to +8°C within specification range for 36months.
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文献和实验Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
with two-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (2DE) to detect changes in the redox proteome of cultured cells. Reduced thiols are labelled with the fluorescent compound 5-iodoacetamidofluorescein. To monitor oxidized thiols
Analysis of methylation using bisulphite sequencing
, since the complexity of DNA is reduced. Therefore I would suggest to select 2-3 pairs of primers, check them on bisulphite modified DNA, and use the most specific ones. Protocol Isolate genomic DNA with the quality sufficient
Respiration: Succinic Dehydrogenase
Materials Mitochondrial suspension from Exercise 8.4 Sodium dithionite 0.2 M Sorenson Phosphate Buffer, pH 7.5 1% (w/v) Bovine Serum Albumin 0.005 M
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