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- 技术资料
- 保存条件：
2-8℃
- 保质期：
24months
- 英文名：
Bovine Serum Albumin (BSA), Fraction V fatty acids ≤0.2 mg/g
- 供应商：
上海汇谱仪器有限公司
- CAS号：
9048-46-8
Use Bovine Serum Albumin (BSA) as a buffering agent, stabilizer, standard
and for blending. Bovine Serum Albumin (BSA) is also a versatile tool against
non-specific solid phase interference. As blocking reagent Bovine Serum
Albumin (BSA)saturates unoccupied binding sites on the solid phase. Use
Bovine Serum Albumin (BSA) typically at a concentration of 0.5 to 3% within
the reagent buffer.
Benefits
■ Keep your diagnostic reagent free from IgG contaminants.
■ Take advantage of the strongly reduced concentration of fatty acids
■ Rely on the proven diagnostic quality of this product.
CAS: 9048-46-8
Properties
Molecular weight: 68 kD
Bovine Serum Albumin (BSA) contains no detectable IgG.
Bovine Serum Albumin (BSA) is controlled for low molecular weight contaminants.
Bovine Serum Albumin (BSA) consists primarily of monomeric albumin.
Specification
Appearance: Slightly yellow lyophilizate
Protein (from N, according to elementary analysis): ≥97%
Water (K. Fischer): ≤5%
Na (flame photometric): ≤0.5%
K (flame photometric): ≤0.01%
Fe (AAS): ≤0.001%
Cu (AAS): ≤0.002%
Fatty acids, total (GC): ≤0.2 mg/g
Triglycerides (enzymatically): Not detectable
Immunoglobulines (ELISA): Not detectable
Country of origin: USA
Stability: At +2 to +8°C within specification range for 24 months.
Remarks:
Official veterinary certificate of health of the donor animals is available.
Official certificate of the deactivation of animal material including the method
(acid treatment at pH 5 for 3 hours) is available.
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文献和实验Metabolic Labeling with Fatty Acids
of radiolabeled fatty acids (e.g., [3 H]myristate or palmitate) is an alternative method for labeling proteins. This unit contains methods for biosynthetic labeling with fatty acids, analysis of the fatty acid linkage with protein, analysis of total protein?bound
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
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