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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
DIG Labeled Control RNA 50ul
- 供应商：
上海汇谱仪器有限公司
- CAS号：
459130-BR
- 规格：
50 µl
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文献和实验DIG (Digoxigenin) labeled RNA probe In situ hybridization protocol
tubes. Heat for 95°C for 2 minutes on a PCR block. This will dehybridize the RNA or DNA probe. Chill on ice immediately to prevent rehybridization. 9. Drain off the hybridization solution. Add 50 to 100 ul of diluted probe per section (ensure
) for nonradioactive labeling of RNA and DNA probes has been developed so that sensitivity is comparable with radioactivity. This chapter will describe the use of DIG-labeled cDNA probes. The use of RNA probes using the DIG system is described in Chapter 47 .
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
DAY 1: 1) Reprecipitate DNA's Add the following DNA's to a 1.5 ml centrifuge tube, mixing with pipet: 20 ug Cot-1 DNA (~20ul) ~200 ng Biotin labeled DNA (~10ul) ~200 ng Digoxigenin labeled DNA (~10ul) Add 1/10th
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