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- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.053 1.981 2.017 1.918
250 1.568 1.545 1.557 1.458
125 0.823 0.811 0.817 0.718
62.5 0.420 0.444 0.432 0.333
31.2 0.273 0.285 0.279 0.180
15.6 0.178 0.182 0.180 0.081
7.8 0.127 0.131 0.129 0.030
0 0.098 0.100 0.099
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 92
Range % 87-96
1:2 Average % 106
Range % 98-109
1:4 Average % 92
Range % 88-95
1:8 Average % 96
Range % 90-101
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
回收率:The recovery of human ADAM10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 84-94
EDTA plasma (n=4) 94 90-98
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文献和实验每孔上样总蛋白量一致，实现样本间公平对比。 三、实战 protocol：肿瘤组织 IFN-γ 检测完整流程 以 “磁控 CAR-T 模拟细胞治疗后，肿瘤组织 IFN-γ 浓度检测” 为例，操作如下： 1.实验准备：试剂（abs9225、abs9161、abs9232、Mouse IFN-γ ELISA Kit 等）、仪器（组织处理器、高速冷冻离心机、酶标仪等）、治疗组与对照组小鼠肿瘤组织。 2. 组织裂解：肿瘤组织离体后用 PBS 冲洗吸干，精准称量 10mg 放入研磨管；加入 100μl 现配
Kit 2.813pg/ml 4.688-300pg/ml (1pg=44mIU) EH0173 Human IL-10 ELISA Kit 4.688pg/ml 7.813-500pg/ml (1ng=14500mIU) QT-EH0173 Human IL-10 QuickTest ELISA Kit 4.688pg/ml 7.813-500pg/ml (1ng=14500mIU) EH0173
Human IL-18 OneStep ELISA Kit abs551019 Human IL-33 ELISA Kit S0C3040 Human IFN-γ OneStep OptiQuant ELISA Kit S0C3058 Human IL-22 OneStep ELISA Kit S0C3050 Human IL-10 OneStep ELISA Kit abs551916 Human IL-25 ELISA Kit UA040005 TNF-α Protein
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