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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.053 1.981 2.017 1.918
250 1.568 1.545 1.557 1.458
125 0.823 0.811 0.817 0.718
62.5 0.420 0.444 0.432 0.333
31.2 0.273 0.285 0.279 0.180
15.6 0.178 0.182 0.180 0.081
7.8 0.127 0.131 0.129 0.030
0 0.098 0.100 0.099
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 92
Range % 87-96
1:2 Average % 106
Range % 98-109
1:4 Average % 92
Range % 88-95
1:8 Average % 96
Range % 90-101
Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit
回收率:The recovery of human ADAM10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 84-94
EDTA plasma (n=4) 94 90-98
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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