Human A Disintegrin And Metall

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected
500 2.053 1.981 2.017 1.918
250 1.568 1.545 1.557 1.458
125 0.823 0.811 0.817 0.718
62.5 0.420 0.444 0.432 0.333
31.2 0.273 0.285 0.279 0.180
15.6 0.178 0.182 0.180 0.081
7.8 0.127 0.131 0.129 0.030
0 0.098 0.100 0.099

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 92
Range % 87-96
1:2 Average % 106
Range % 98-109
1:4 Average % 92
Range % 88-95
1:8 Average % 96
Range % 90-101

Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit

回收率:
The recovery of human ADAM10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 84-94
EDTA plasma (n=4) 94 90-98