Mouse Disintegrin and metallop

Mouse Disintegrin and metalloproteinase domain-containing protein 17(ADAM17) ELISA kit

标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected
1000 2.404 2.601 2.503 2.335
500 1.632 1.589 1.611 1.443
250 0.977 0.989 0.983 0.815
125 0.606 0.588 0.597 0.429
62.5 0.356 0.342 0.349 0.181
31.25 0.258 0.278 0.268 0.100
15.6 0.202 0.215 0.209 0.041
0 0.161 0.174 0.168

Mouse Disintegrin and metalloproteinase domain-containing protein 17(ADAM17) ELISA kit

精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Mouse Disintegrin and metalloproteinase domain-containing protein 17(ADAM17) ELISA kit

线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAM17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 86
Range % 83-89
1:2 Average % 94
Range % 89-99
1:4 Average % 103
Range % 97-109
1:8 Average % 99
Range % 95-103

Mouse Disintegrin and metalloproteinase domain-containing protein 17(ADAM17) ELISA kit

回收率:
The recovery of mouse ADAM17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 89 83-95