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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
100只/盒
- 供应商:
研卉生物
- 现货状态:
6-10周
- 保修期:
VS2062
- 规格:
12只
- 1000KD超滤管赛多利斯产品信息
- Vivaspin 6,1000000 MWCO PES,100件
Vivaspin® 6可以在适合50mL锥形底离心管的离心机中对30μL至6mL体积的样品进行浓缩,当您对抗体、蛋白质、纳米微粒以及病毒样品进行浓缩时, Vivaspin® 6会是您的最佳选择。 Vivaspin® 6采用双排垂直膜设计,其中的优质PES垂直膜可以加快样品的浓缩速度并提高样品回收率。内置的死端结构收集器能够确保安全、完全的浓缩液回收。Vivaspin® 6是唯一一款大小适用于对6mL样品进行操作的装置。
产品编号: VS0662 - High flux PES membrane
- High retentate recovery > 90 %
- Vertical twin membrane for ultra fast concentration
- Universal rotor compatibility
- Safe sample concentration due to dead-stop volume
- Convenient concentrate recovery by pipetting
- Volume graduation for easy reading/monitoring
- 100-fold concentration with twin vertical membranes
- Additional option for pressurized operation
Description
Vivaspin 20 centrifugal concentrators have been developed to offer increased volume flexibility and performance. Vivaspin 20 handles up to 20 ml in swing bucket rotors and 14 ml in fixed angle rotors accepting 50 ml centrifuge tubes. Vivaspin 20 features twin vertical membranes for unparalleled filtration speed and >100-fold concentration. Volume graduation allows to monitor the concentration process and the modified dead stop pocket simplifies pipetting of the final concentrate. For higher process flexibility, Vivaspin 20 is available with unique accessories and operating methods. For one-step desalting following concentration, a diafiltration cup filled with buffer is placed on the concentrator and spun another time to achieve 98% salt removal, instead of two spins to achieve the same result with the traditional refill and re-spin procedure. When an appropriate centrifuge is unavailable, or for single sample processing, for gas pressure filtration, Vivaspin 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, gas pressure filtration can be combined with centrifugation. "Pressure-fugation" is particularly useful for difficult or viscous samples such as serum, when using a low process temperature, or generally when minimum process time is essential.
Rotor Compatibility
Please note: Vivaspin 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus/Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes. These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20. If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin 15, which can be used in either round bottom or conical centrifuge tubes
Performance Characteristics
| Time to concentrate up to 30x [min.] at 20°C and solute recovery % | ||||
|---|---|---|---|---|
| Mode | Centrifuge | Centrifuge | Bench top | Press-fuge |
| Rotor | Swing bucket | 25° Fixed angle | Pressure | Swing bucket |
| Sart volume | 20 ml | 14 ml | 10 ml | 10 ml |
| Min./Rec. | Min./Rec. | Min./Rec. | Min./Rec. | |
| Cytochrome c 0.25 mg/ml (12,400 MW) | ||||
| 3,000 MWCO PES | 110 / 97 % | 180 / 96 % | 60 / 96 % | - / - |
| BSA 1.0 mg/ml (66,000 MW) | ||||
| 5,000 MWCO PES | 23 / 99 % | 29 / 99 % | 50 / 98 % | 14 / 98 % |
| 10,000 MWCO PES | 16 / 98 % | 17 / 98 % | 32 / 97 % | 8 / 97 % |
| 30,000 MWCO PES | 13 / 98 % | 15 / 98 % | 32 / 97 % | 8 / 97 % |
| IgG 0.25 mg/ml (160,000 MW) | ||||
| 30,000 MWCO PES | 27 / 97 % | 20 / 95 % | 46 / 94 % | 13 / 97 % |
| 50,000 MWCO PES | 27 / 96 % | 22 / 95 % | 46 / 93 % | 13 / 96 % |
| 100,000 MWCO PES | 25 / 91 % | 20 / 90 % | 42 / 88 % | 12 / 94 % |
| Latex beads 0.004% in DMEM +10% FCS (0.055 μm) | ||||
| 300,000 MWCO PES | 20 / 99 % | 35 / 99 % | 10 / 99 % | - / - |
| Latex beads 0.004% in DMEM +10% FCS (0.24 μm) | ||||
| 1,000,000 MWCO PES | 4 / 99 % | 12 / 99 % | 4 / 99 % | - / - |
| Yeast 1.0 mg/ml (S. Cerevisiae) | ||||
| 0.2 μm PES | 15 / 95 % | 5 / 95 % | 20 / 95 % | 2 / 95 % |
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文献和实验的复杂了,更不利于后期的研究了。 第三步初步浓缩(也可省略此步):是不是在提取干细胞上清外泌体过程中会有看不到沉淀的时候?这时心中就没了谱,下游的检测项目做的更是提心吊胆。因此,在这里给出了通过超滤方法进行浓缩处理的建议。超滤的原理就是利用一定的尺寸大小的滤膜拦住大颗粒物而放过小颗粒物的原理,在离心管中最终呈现上液和下液,我们在根据实验目的各取所需。针对细胞上清液的特点,建议可以使用 10 KD ~ 100 KD 之间的超滤管来进行样品浓缩,外泌体通常是在超滤离心管的上室中,浓缩倍数控制到 5 倍
什么样提取方法都很难看到外泌体沉淀,心中就没了谱,下游的检测项目做的更是提心吊胆。因此,在这里给出了通过超滤方法进行浓缩处理的建议。超滤的原理就是利用一定的尺寸大小的滤膜拦住大颗粒物而放过小颗粒物的原理,在离心管中最终呈现上液和下游,我们在根据实验目的各取所需。针对细胞上清液的特点,建议可以使用 10 KD ~ 100 KD 之间的超滤管来进行样品浓缩,外泌体通常是在超滤离心管的上室中,浓缩倍数控制到 5 倍左右;针对干细胞、神经细胞等可以将浓缩倍数提升到 10 倍左右,因为这些细胞外泌体分泌量相对肿瘤
【资源】蛋白版2006年05月无人应助帖,请广大战友积极应助,加分从优!
://www.dxy.cn/bbs/post/view?bid=65&id=6169849&sty=1&tpg=18&age=0 【求助】1000kd的蛋白怎么转啊 http://www.dxy.cn/bbs/post/view?bid=65&id=6166313&sty=1&tpg=18&age=0 【求助】目的条带位置不正确 http://www.dxy.cn/bbs/post/view?bid=65&id=6165184&sty=1&tpg=18&age=0 【求助】有谁知道哪有“氧代肉豆
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