- ￥85
- pall
- 美国
- MAP003C38
- 2025年07月14日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
100支
- 供应商：
研卉生物
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有
- 规格：
支
原价102/支，80元/支，卖完即止。
Quickly Concentrates Up To
20 mL of Biological Sample
- Rapidly concentrates 20 mL sample volumes to 0.5 mL
- Provides high recoveries, typically > 90%
- Low protein-binding Omega™ membrane and polypropylene housing minimize losses due to non-specific binding
- Versatile Omega membrane is available in a variety of MWCOs
- Built-in deadstop prevents spinning to dryness
- Color-coded for easy identification
-
Ultrafiltration
- Concentrate and desalt proteins
- Exchange buffer or remove salt of chromatography eluates and gradient fractions
- Recover proteins or other molecules from cell culture supernatants
-
Microfiltration
- Remove particulate from aqueous solutions and clinical samples
-
Materials of Construction
- Filter Media: Omega (modified polyethersulfone) and Supor® (polyethersulfone) membranes
- Sample Reservoir, Filtrate Receiver, and Cap: Polypropylene
- Paddle: Polyethylene
-
Effective Filtration Area
- 7.2 cm2
-
Dimensions
- Diameter: 29 mm (1.2 in.)
- Length: 12.0 cm (4.7 in.)
-
Operating Temperature Range
- 0 - 40 °C (32 - 104 °F)
-
Capacities
- Maximum Sample Volume: 20 mL
- Final Concentrate Volume:
450 μL (swinging bucket)
1.2 - 1.5 mL (45º angle rotor)
1.5 mL (34º angle rotor) - Filtrate Receiver Volume: 22 mL
- Hold-Up Volume: 80 μL (membrane and paddle)
-
pH Range
- 1 - 14
-
Maximum Centrifugal Force
- 5,000 x g (ultrafiltration)
- 14,000 x g (microfiltration)
-
Centrifuge
- Fits centrifuges that accept standard 50 mL conical end tubes
-
Sanitization
- Provided non-sterile. May be sanitized by filtering 70% ethanol through thedevice prior to use.
-
Macrosep Advance Centrifugal Devices: Reduced Spin Time
Protein solutions were processed in each of the Macrosep Advance devices. Average time (minutes) is plotted against mL of remaining product to be filtered using a swinging bucket rotor at 5,000 x g. Solutions are 3K: Protamine Sulfate, 0.1% in 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.
Macrosep Advance Centrifugal Devices: Retention Efficiency
Protein solutions were processed in each of the Macrosep Advance devices. Average percent retention using a swinging bucket rotor at 5,000 x g is displayed for each MWCO. Solutions were 3K: Protamine Sulfate, 0.1% 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.
Macrosep Advance Centrifugal Devices with Omega Membrane MAP001C36 1K, yellow 6/pkg MAP001C37 1K, yellow 24/pkg MAP001C38 1K, yellow 100/pkg MAP003C36 3K, gray 6/pkg MAP003C37 3K, gray 24/pkg MAP003C38 3K, gray 100/pkg MAP010C36 10K, blue 6/pkg MAP010C37 10K, blue 24/pkg MAP010C38 10K, blue 100/pkg MAP030C36 30K, red 6/pkg MAP030C37 30K, red 24/pkg MAP030C38 30K, red 100/pkg MAP100C36 100K, clear 6/pkg MAP100C37 100K, clear 24/pkg MAP100C38 100K, clear 100/pkg Macrosep Advance Centrifugal Devices with Supor Membrane MAPM02C67 0.2 μm, aqua 24/pkg MAPM02C68 0.2 μm, aqua 100/pkg MAPM45C67 0.45 μm, wildberry 24/pkg MAPM45C68 0.45 μm, wildberry 100/pkg
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文献和实验用物理方法处理掉? 建议在你的样品上超滤前选用0.45um的膜过滤一下，这样就不会有这个问题了，长时间有大颗粒或别的杂质进去对膜包可不太好。 (四)问题：我的蛋白14kD，用截流10000的怎么一点都离不下。而且在我只加超纯水时，水也离不下，十分迷惑 你是用超滤离心管吗?水都离心不下，不是离心力不够吧，要不膜用别的什么处理过了变疏水的了。还是选错了型号。 (五)问题：请问Millipore 的超滤管可以反复使用吗? 如果可以的话,第一次使用
用物理方法处理掉? 建议在你的样品上超滤前选用0.45um的膜过滤一下，这样就不会有这个问题了，长时间有大颗粒或别的杂质进去对膜包可不太好。 (四)问题：我的蛋白14kD，用截流10000的怎么一点都离不下。而且在我只加超纯水时，水也离不下，十分迷惑 你是用超滤离心管吗?水都离心不下，不是离心力不够吧，要不膜用别的什么处理过了变疏水的了。还是选错了型号。 (五)问题：请问millipore的超滤管可以反复使用吗? 如果可以的话,第一次使用后该如何处理
烷基硫酸钠（SDS）；三羟甲基氨基甲烷（Tris）；乙二胺四乙酸（EDTA）；饱和酚；氯仿；异戊醇；无水乙醇；75%乙醇；蛋白酶K；RNase酶；手术剪刀、镊子、吸水纸；微量取液器；研钵、1.5mL 离心管、一次性手套、1.5mL离心管架、记号笔…… 试剂配制 1．Tris-HCL 1mol/L PH8.0 50ml 配制方法：40ml双蒸水，6.057g固体Tris放入烧杯中溶解，用浓盐酸调PH值到8.0，转移到50ml容量瓶中，加入双蒸水定容，摇匀后，转到准备好的输液瓶中，贴上标签，高压灭菌后，降至室温
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