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- PEPROTECH
- 中国/美国
- xy-240Hu01
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Secreted Ly6/uPAR Related Protein 1 (SLURP1)
- 库存：
1000
- 供应商：
上海信裕
分泌型Ly6/uPAR相关蛋白1(SLURP1)重组蛋白
Recombinant Secreted Ly6/uPAR Related Protein 1 (SLURP1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | xy240Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 15.0kDa |
| Concentration | 6.7 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
分泌型Ly6/uPAR相关蛋白1(SLURP1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MASRWAVQLL LVAAWSMGCG EALKCYTCKE PMTSASCRTI TRCKPEDTAC MTTLVTVEAE YPFNQSPVVT RSCSSSCVAT DPDSIGAAHL IFCCFRDLCN SEL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MASRWAVQLL LVAAWSMGCG EALKCYTCKE PMTSASCRTI TRCKPEDTAC MTTLVTVEAE YPFNQSPVVT RSCSSSCVAT DPDSIGAAHL IFCCFRDLCN SEL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
分泌型Ly6/uPAR相关蛋白1(SLURP1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
分泌型Ly6/uPAR相关蛋白1(SLURP1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
分泌型Ly6/uPAR相关蛋白1(SLURP1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
时限短暂；瞬时表达系统的优点是简捷，实验周期短。大规模的瞬时表达技术是 近年来的一个研究热点。已有报道能放大到100 L反应器中生产重组蛋白，产量(分泌型蛋白)可达l~10 mg/L。不过该方法技术条件要求高，如质粒的纯度、转染的效率等。 稳定表达系统是指载体进入宿主细胞并经选择培养，载体DNA稳定存在于细胞内，目的蛋白的表达持久、稳定。由于需抗性选择甚至加压扩增等步骤，稳定表达相对耗时耗力。Geisse等以白血病抑制因子的制备为例，比较了几种不同稳定表达体系在产量及时间上的差异(表 3)，把目的
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分泌型Ly6/uPAR相关蛋白1(SLURP1)重组蛋白
¥1 - 3980
