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- PEPROTECH
- 中国/美国
- xy-910Ra01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Kallikrein 7 (KLK7)
- 库存：
1000
- 供应商：
上海信裕
激肽释放酶7(KLK7)重组蛋白
Recombinant Kallikrein 7 (KLK7)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy910Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 57.5kDa |
| Concentration | 6.1 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
激肽释放酶7(KLK7)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.05% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-RVIGGYK CEKNSQPWQV ALYSFTKYLC GGVLIDPSWV ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD YKPFLMRNHT RKPGDDHSNDLMLLHLSQPA DITDGVKVID LPTEEPKVGS TCLASGWGST KPLIWEFPDD LQCVNIHLLS NEKCIKAYKE KVTDLMLCAG ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP AIYTKLIKFT SWIKE
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-RVIGGYK CEKNSQPWQV ALYSFTKYLC GGVLIDPSWV ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD YKPFLMRNHT RKPGDDHSNDLMLLHLSQPA DITDGVKVID LPTEEPKVGS TCLASGWGST KPLIWEFPDD LQCVNIHLLS NEKCIKAYKE KVTDLMLCAG ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP AIYTKLIKFT SWIKE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
激肽释放酶7(KLK7)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
激肽释放酶7(KLK7)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
激肽释放酶7(KLK7)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
-Triton蛋白分级抽提试剂盒细胞膜NP-40 or RIPA膜蛋白抽提试剂盒蛋白分级抽提试剂盒细胞核RIPA核蛋白抽提试剂盒线粒体RIPA线粒体蛋白抽提试剂盒亚细胞定位蛋白抽提试剂盒蛋白酶磷酸酶抑制剂抑制剂蛋白酶或磷酸酶PMSF丝氨酸蛋白酶抑制剂，如胰凝乳蛋白酶、胰蛋白酶和凝血酶，也抑制半胱氨酸蛋白酶如木瓜蛋白酶Leupeptin 亮肽素抑制丝氨酸和半胱氨酸蛋白酶，如胰蛋白酶、木瓜蛋白酶、纤溶酶和组织蛋白酶BAprotinin 抑肽酶丝氨酸蛋白酶抑制剂，抑制纤维蛋白溶酶、激肽释放酶、胰蛋白
洲8个国家的生产厂商，应用此项技术工业规模化生产口蹄疫疫苗和兽用狂犬疫苗，已掌握了5000L的细胞罐大规模培养技术。 目前已实现商业化的产品有：口蹄疫疫苗、狂犬病疫苗、牛白血病病毒疫苗、脊髓灰质炎病毒疫苗、乙型肝炎疫苗、疱疮病毒疫苗、巨细胞病毒疫苗、α及β干扰素、血纤维蛋白溶酶原激活剂、凝血因子Ⅷ和Ⅸ、促红细胞素、松弛素、生长激素、蛋白C、免疫球蛋白、尿激酶、激肽释放酶及200种单克隆抗体等。其中，口蹄疫疫苗是动物细胞大规模培养方法生产的主要产品之一。1983年，英国Wellcome公司就已能够利用
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激肽释放酶7(KLK7)重组蛋白
¥1 - 3980
