Recombinant Human Soluble CD4
Source Baculovirus
Background CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MHC (Major Histocompatibility Complex) Class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as HIV receptors, binding directly to the envelope protein gp120 on HIV. Thymocytes which are CD4 (+)and CD8 ( - )produce factors such as IL-4 , IL-5 ,IL-10 and IFN-gamma.
Description Recombinant Human sCD4 produced in Baculovirus is a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 40,000 Dalton. As a result of glycosilation the sCD4 migrates as a 46,000 dalton protein on standard SDS-PAGE. Detected By Western Imunno Blots anti cd4 monoclonal antibody.
Recombinant soluble CD4 is purified by proprietary chromatographic techniques.
Physical Appearance Sterile Filtered colorless liquid formulation.
Recombinant Human Soluble CD4Formulation Recombinant Human Soluble CD-4 solution contains 10mM sodium phosphate buffer pH-7.2, 300mM sodium chloride and 0.01% tween.
Stability rHusCD4 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Recombinant Human Soluble CD4Dimers and aggregates Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity CHI’s Recombinant Human Soluble CD-4 is fully biologically active when compared to standard. Immune reactive in ELISA using monoclonal anti CD4 in gpl2O capture ELISA assays.
Endotoxin Less than 0.1 ng/µg (IEU/µg) of soluble CD4.
Protein content Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of CD4 as a Reference Standard.
Recombinant Human Soluble CD4Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.