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Recombinant Human Thyroid Pero

xidase
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  • ¥1680 - 3988
  • 美国ATCC
  • 美国/中国
  • 2025年12月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 供应商

      钰博生物

    • 细胞类型

      复苏

    • 组织来源

      见说明书

    • 物种来源

      人、大鼠、小鼠、、、、

    • 免疫类型

      请咨询销售人员

    • 细胞形态

      上皮样;多角形

    • 是否是肿瘤细胞

      见说明书

    • 器官来源

      见说明书

    • 运输方式

      干冰运输or活细胞运输

    • 年限

      见说明书

    • 生长状态

      贴壁生长

    Recombinant Human Thyroid Peroxidase
    Synonyms Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.
    Introduction Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.
    Recombinant Human Thyroid PeroxidaseDescription Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass.
    The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
    Source Sf9 insect cells.
    Formulation TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.
    Immunological functions 1. Binds IgG-type human auto-antibodies.
    2. Auto-antibodies to TPO recognize conformation-dependent epitopes.
    3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).
    Recombinant Human Thyroid PeroxidaseApplications Western-Blot with monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody.
    Coating concentration 0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.
    Storage Conditions Store at 4℃ if entire vial will be used within 2-4 weeks.
    Store, frozen at -20℃ for longer periods of time.
    Avoid multiple freeze-thaw cycles.
    Purity Greater than 95% as determined by SDS-PAGE.
    Recombinant Human Thyroid PeroxidaseUsage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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    图标文献和实验
    相关实验
    • Human Thyroid Culture

      The technique described in this chapter enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland. Human thyroid cells

    • Human Thyroid Epithelial Cells

      The thyroid gland contains two populations of epithelial cells, of quite different embryological origin and function. Only the major component—the follicular cells—will be considered here. The minor C cell population, which forms

    • Transfection and Transformation of Human Thyroid Epithelial Cells

      cells, but again is applicable only to cloned genes of restricted size, and safety considerations are likely to restrict greatly the use of retroviral vectors suitable for human cells. Calcium phosphate coprecipitation has been used with success

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