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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human skeletal muscle myosin purified from myofibrils.
- 亚型:
Mouse IgG1
- 形态:
液态
- 保存条件:
保存于-20℃
- 克隆性:
单克隆
- 标记物:
无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)
- 适应物种:
Hu, Mo, Rab, Rat
- 保质期:
按说明书条件可保存1-2年
- 抗原来源:
见详细说明资料
- 目录编号:
PL0401263
- 级别:
超纯
- 库存:
大量
- 供应商:
华拓生物
- 宿主:
Mouse
- 应用范围:
WB: 1:200-500 IHC: 1:100-200
- 浓度:
1mg/ml
- 靶点:
Myosin(slow)
- 抗体英文名:
Myosin(slow) Antibody
- 抗体名:
Myosin(slow)单克隆抗体
- 规格:
0.1ml
Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)
Background:
This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
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文献和实验,肌球蛋白(myosin)单克隆抗体二、实验器械1、培养皿2、培养瓶3、直剪和眼科剪4、眼科镊和止血钳5、烧杯6、15ml离心管三、实验流程取肌肉于平皿,Hank’s洗3次↓剔除脂肪、结缔组织↓肌肉标本剪约0.1cm3小块↓移至离心管,Hank’s洗3次↓静置1min,弃去上层液及漂浮组织↓0.25%胰酶,37度水浴消化20min,每5min摇动或吹打1次↓生长培养基终止消化,反复吹打,依次100,200,400目过筛↓离心,1000rmp,10min,弃去上清↓重悬,计数细胞,接种密度以5 ×
Determination of Muscle Fiber Type in Rodents
consist of muscle fibers that can differ in both composition and functional characteristics. These three types of muscle fibers, broadly categorized as slow, fast IIa, and fast IIb muscle fibers, express characteristic myosin heavy chain proteins
the cell body forward and release of the attachmentsat the rear, as the cell retracts, completes the cycle. Slow-moving cells, suchas fibroblasts, show these distinct steps of migration, but they are less obviousin other cell types. For example, rapidly
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