Recombinant Porcine Interleuki

Synonyms Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), ), IL-1 alpha, IL1, IL-1A, IL1F1.

Introduction Interleukin-1 alpha is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. Circulating levels of are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1 alpha possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.

Recombinant Porcine Interleukin-1 alphaDescription Interleukin-1A Porice Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton.

The IL-1A is purified by proprietary chromatographic techniques.

Source Escherichia Coli.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Recombinant Porcine Interleukin-1 alphaPurity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser.

Biological Activity The ED50 as determined by the dose-dependant stimulation of D10S cells is < 0.03 ng/ml corresponding to a specific activity of 33.4MIU/mg.

Protein content Protein quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.

Recombinant Porcine Interleukin-1 alphaUsage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.