- 询价
- PEPROTECH
- 中国/美国
- xy-511Hu01
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Heat Shock 105kDa/110kDa Protein 1 (HSPH1)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Homo sapiens (Human) |
| Product No. | xy511Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 14.8kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGHHHHHHSGSEF-KVR EFSVTDAVPF PISLIWNHDS EDTEGVHEVF SRNHAAPFSK VLTFLRRGPF ELEAFYSDPQ GVPYPEAKIG RFVVQNVSAQ KDGEKSRVKV KVRVNTHGIF TISTASMVEK VPTEE
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
105kDa/110kDa热休克蛋白1(HSPH1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Size Price (RMB) AL0103050 (6 x 50ųl) 6,042.68 AL0103200 (6 x 200ųl) 19, 752.40 AL0103500 (6 x 500ųl) 45,092.65 CFPE700-新一代无细胞蛋白表达试剂盒（小麦胚芽） 新一代无细胞蛋白质表达试剂盒（小麦胚芽）是一种低成本、高灵活性、高产量重组蛋白表达系统，采用便捷的protocol可在一天
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择： 尿素和盐酸胍属中强度变性剂，易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用，所需浓度尿素8-10M，盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱，溶解度为70-90%，尿素在作用时间较长或温度较高时会裂解形成氰酸盐，对重组蛋白质的氨
，使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择： 尿素和盐酸胍属中强度变性剂，易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用，所需浓度尿素8-10M，盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱，溶解度为70
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