- ¥385
- sigma
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- 10771-100ML
- 2025年11月01日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 英文名：
Histopaque®-1077
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.General descriptionA solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. This medium facilitates the recovery of large numbers of viable mononuclear cells.法律信息Histopaque 为以下机构的注册商标： Sigma-Aldrich Co. LLC
性质
Related CategoriesDensity Gradient Media, Histopaque, Histopaque and Nonionic Iodinated Density Gradient Media, 细胞生物学, 血液学和组织学 更多...Quality Level PREMIUMsterility sterile-filteredshelf life Expiry date on the label.impurities endotoxin, testeddensity 1.077 g/mLstorage temp. 2-8°C
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文献和实验).Transfer serum/white blood cells to 50 ml tube, underlay 12 ml RT Histopaque 1077.Spin 2500 rpm/30min at RT. To take mononuclear cells:Take off serum above band. (See attached figure, in preparation)Take mononuclear band + some Ficoll into two 15ml tubes
Purification of human mononuclear cells and neutrophils
citrate/citric acid. Fill to 60 ml total (blood 43 ml). Mix and sediment 30 min at RT (white blood cells now with serum). Transfer serum/white blood cells to 50 ml tube, underlay 12 ml RT Histopaque 1077. Spin 2500 rpm/30min at RT.
分离人单核细胞和中性粒细胞-Purification of human mononuclear cells and neutrophi
Histopaque 1077 18 ml cold H2 O 2 ml 10x PBS M199 for HUVECs: 1L powder pocket M199 + 35g NaHCO3 + 25mM Hepes + 5 mM glutamine + 50 ug/ml Gentamycin. 1M Hepes: 119.1 g Hepes + 500 ml dH2 O. Media A: 1X HBSS (10X:50ml) + 10
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