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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Interleukin 21 (IL21)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy688Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
白介素21(IL21)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验ERLKSLIQKM IHQHLS2, FeaturesDL-IL21-Hu人白介素 21 (IL21) 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL21 含量。标本的采集与保存1. 血清:将收集于血清分离管的全血标本在室温放置 2 小时或 4℃ 过夜,然后 1000×g 离心 20 分钟,取上清即可,或将上清置于-20℃ 或-80℃ 保存,但应避免反复冻融。2. 血浆:用 EDTA 或肝素作为抗凝剂采集
重组蛋白纯化基本原则蛋白的分离纯化是生物工程下游阶段一个比较重要的部分,尤其在基因工程重组蛋白的分离纯化中,上游过程的许多因素会直接影响到下游蛋白的分离,充分利用上游对下游的影响,对蛋白的纯化做一个全面的考虑和整体的设计。是否带有亲和标签,His标签,GST标签等,不同的亲和标签选择不同的纯化方案;可能是可溶性表达,可能形成包涵体,可溶性的蛋白往往需要复杂的纯化步骤,而包涵体易于分离,纯度较高,但回收具有生物活性的蛋白却变的相当困难,需要对聚集的蛋白进行变复性,通常活性蛋白的得率比较低;是否
/ml,不可振荡。 这个步骤即为溶解步骤,非常重要。 1) 一定要用推荐的溶液重悬(或溶解)冻干粉 用于溶解细胞因子或蛋白的溶液千差万别。此例中的重组人IL-4需用水溶解,而重组人IL-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解
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