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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant High Mobility Group Box Protein 2 (HMGB2)
- 库存：
1000
- 供应商：
上海信裕
高迁移率族框蛋白2(HMGB2)重组蛋白
Recombinant High Mobility Group Box Protein 2 (HMGB2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 24.7kDa |
| Concentration | 9.9 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Gly2~Pro187 (Accession # P26583) with two N-terminal Tags, His-tag and T7-tag |
高迁移率族框蛋白2(HMGB2)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS AFFLFCSEHR PKIKSEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEP
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS AFFLFCSEHR PKIKSEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
高迁移率族框蛋白2(HMGB2)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
高迁移率族框蛋白2(HMGB2)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
检测实验，或用相应激酶（见注释 3 ) 的已知底物在溶液中对激酶的活性进行检测。如果在文献中未查到激酶的最佳活化条件，那么每种激酶的最优活化条件（如最佳缓冲液条件、激酶的浓度、激酶的孵育时间）要通过实验来确定。 ( 3 ) 建议使用一种阳性对照（如该激酶的一个已知底物）。在研究大麦 CK2α 实验中 [22]，我们用阳性对照来区别大麦蛋白，它们和不同植物 HMG ( 高迁移率群）的蛋白质具有很高的同源性，这些蛋白质就是众所周知的 CK2α 靶蛋白 [28，29] 。在另一项研究实验中
研究，其中包括了基因工程单链抗体（single chain Fv，scFv）的应用。一般抗体均大于150KD，不易渗透到组织中，而鼠源抗体用于人体易产生人抗鼠Ig抗体。应用基因工程技术产生的单链抗体是由Ig重链可变区（VH）和轻链可变区（VL）通过一段小肽连接在一起的重组蛋白。分子量仅为27KD，免疫原性降低，易于进入瘤组织，血中半衰期短，易于清除，无Fc段不会与具有Fc受体的正常细胞结合，并且易于产业化。为了提高显像效果Nieroda 用生物反应调节剂 g-干扰素与同位素标记抗体同时使用，促使肿瘤
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高迁移率族框蛋白2(HMGB2)重组蛋白
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