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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Protein Kinase, AMP Activated Beta 1 (PRKAb1)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy671Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
AMP激活蛋白激酶β1(PRKAβ1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Ribonuclease Protection Assay for the Detection of β1-Adrenergic Receptor RNA
epinephrine and norepinephrine; when ligand-activated, these receptors initiate the production of cyclic AMP. Three subtypes of β-ARs, β1 -AR, β2 -AR, and β3 -AR, have been identified on the basis of their pharmacological properties, physiological effects
。抗原激活的T细胞和LPS激活的单核/巨噬细胞分泌具有生物学活性的TGF-β1。TGF-β1的作用显示多功能性,表现为可抑制很多类型细胞的生长,刺激另一些细胞的生长;而且在不同的培养条件下,对于同一类型细胞TGF-β1既能表现抑制作用也能表现刺激作用。TGF-β能刺激胞外基质蛋白(如胶原)、基质蛋白受体(如整合素)和基质修饰酶(如基质金属蛋白酶)的合成。功能上,TGF-β还拮抗淋巴细胞反应,抑制淋巴细胞增殖,抑制CTL成熟,抑制Mφ的激活,抑制促炎症反应性细胞因子的作用,并作为一种信号可关闭免疫
练的是肌肉,为啥全身都受益?新研究揭示运动激活全身代谢的关键
的一种蛋白在肝脏中激活重要的细胞「自噬」过程,使肝脏的代谢功能更强。 根据论文,研究人员在实验小鼠运动前后对它们的血液进行了蛋白质组学分析,由此发现,运动诱导骨骼肌分泌一种叫做纤维粘连蛋白(FN1)的可溶性蛋白质。 而这些 FN1 蛋白随着血液循环到达肝脏后,通过肝细胞表面的一种受体整合素 α5β1 发出信号,在肝脏中激活「细胞自噬」过程,促进代谢适应。细胞自噬,简单来说就是细胞内的一种物质回收机制,降解和清除细胞内多余的蛋白质或受损的细胞器等。因此,运动在肝脏中激活细胞自噬也意味着这个代谢器官
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