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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Alanine Glyoxylate Aminotransferase (AGXT)
- 库存：
1000
- 供应商：
上海信裕
丙氨酸乙醛酸转氨酶(AGXT)重组蛋白
Recombinant Alanine Glyoxylate Aminotransferase (AGXT)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 25.1kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Thr182~Gly371 with two N-terminal Tags, His-tag and T7-tag |
丙氨酸乙醛酸转氨酶(AGXT)重组蛋白
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
SEQUENCES
The sequence of the target protein is listed below.
TGVLQPLDG FGELCHRYQC LLLVDSVASL GGVPIYMDQQ GIDILYSGSQ KVLNAPPGIS LISFNDKAKS KVYSRKTKPV SFYTDITYLS KLWGCEGKTR VIHHTLPVIS LYCLRESLAL ISEQGLENSW RRHREATAHL HKCLRELGLK FFVKDPEIRL PTITTVTVPA GYNWRDIVSY VLDHFNIEIS G
TGVLQPLDG FGELCHRYQC LLLVDSVASL GGVPIYMDQQ GIDILYSGSQ KVLNAPPGIS LISFNDKAKS KVYSRKTKPV SFYTDITYLS KLWGCEGKTR VIHHTLPVIS LYCLRESLAL ISEQGLENSW RRHREATAHL HKCLRELGLK FFVKDPEIRL PTITTVTVPA GYNWRDIVSY VLDHFNIEIS G
USAGE
丙氨酸乙醛酸转氨酶(AGXT)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
丙氨酸乙醛酸转氨酶(AGXT)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
致性上。细胞培养环境细胞培养环境中抑制因素的积聚是提高细胞密度的主要限制因素。体外动物细胞培养中氨离子的积累是抑制细胞生长的主要因素之一。氨的积聚使细胞内UDP氨基己糖(UDP-N-乙酰葡糖胺和UDP-N-乙酰半乳糖胺)增加，影响细胞的生长及蛋白的糖基化过程。氨抑制Gln代谢途径，使Asp和Glu消耗增加。细胞消耗Asp增加，可能是细胞线粒体膜上苹果酸－天冬氨酸泵转运NADH加快，使细胞维持糖酵解途径的需要。Asp消耗增加可能会从Gln代谢多经天冬氨酸转氨酶途径而不是丙氨酸转氨酶或谷氨酸脱氢酶途径得以补偿
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丙氨酸乙醛酸转氨酶(AGXT)重组蛋白
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