相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Octamer Binding Transcription Factor 4 (OCT4)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy424Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
八聚体结合转录因子4(OCT4)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验摘要 Groundbreaking work demonstrated that ectopic expression of four transcription factors, Oct4, Klf4, Sox2, and c-Myc, could reprogram murine somatic cells to induced pluripotent stem cells (iPSCs) (Takahashi and Yamanaka, 2006), and human
比较常见的。在不同的细胞环境下,某个转录因子经历不同的修饰(如磷酸化和去磷酸化,或者通过与其它蛋白的结合),可获得不同的转录活性。在真核细胞中,抑制或激活难以由单一蛋白来实现,通常依靠“团队”复合体来完成。 但是难于理解的地方在于:在同样的胞内环境中,同样的转录因子(如上面提到的Oct4)行使了相反的功能。如下图。 个人想到的解释是: 1: 转录因子与DNA序列的结合改变了自身构象,从而改变了其对沉默或激活复合物的亲和力; 2: 与其它转录因子的协同作用决定是激活
四因子作用那么,是否可以直接应用相关转录因子对体细胞进行重编程而逆转终未分化细胞的发育潜能, 进而表现出ES细胞那样的多潜能性呢?Takahashi和Yamanaka{4}选择24种在小鼠早期胚胎ES细胞或者肿瘤细胞中丰富表达的转录因子, 通过筛选、组合, 发现用Oct4, Sox2, c-Myc和Klf4 4个因子(这4个因子也被称为Yamanaka因子)可以有效地将胎鼠成纤维细胞(mouse embryonic fibroblast, MEF细胞)和小鼠尾尖成纤维细胞诱导形成形态和生长
技术资料暂无技术资料 索取技术资料






