- 询价
- PEPROTECH
- 中国/美国
- xy-D859Hu01
- 2025年12月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Recombinant Branched Chain Aminotransferase 2, Mitochondrial (BCAT2)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Homo sapiens (Human) |
| Product No. | xy-D859Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 36.1kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | 36.1kDa with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
AFKG KDQQVRLFRP WLNMDRMLRS AMRLCLPSFD KLELLECIRR LIEVDKDWVP DAAGTSLYVR PVLIGNEPSL GVSQPTRALL FVILCPVGAY FPGGSVTPVS LLADPAFIRA WVGGVGNYKL GGNYGPTVLV QQEALKRGCE QVLWLYGPDH QLTEVGTMNI FVYWTHEDGV LELVTPPLNG VILPGVVRQS LLDMAQTWGE FRVVERTITM KQLLRALEEG RVREVFGSGT ACQVCPVHRI LYKDRNLHIP TMENGPELIL RFQKELKEIQ YGIRAHEWMF PV
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验三句话读懂一篇 CNS：让人产生幻觉的毒蘑菇，竟能治疗抑郁症？韩春雨开发 RNA 高效追踪平台
/60），进而证明接种新冠加强针的合理性！ 图 4：来源 Nature 5. Nature Metabolism：支链氨基酸促进肥胖的机制及其靶向干预 肥胖不仅影响外型美观，还破坏身体健康。 2022 年 1 月 24 日，复旦大学雷群英、黄海艳和尹淼联合在 Nature Metabolism 杂志发表了研究论文 BCAA–BCKA axis regulates WAT browning through acetylation of PRDM16。 该研究发现 BCAT2（白色脂肪中
致性上。细胞培养环境细胞培养环境中抑制因素的积聚是提高细胞密度的主要限制因素。体外动物细胞培养中氨离子的积累是抑制细胞生长的主要因素之一。氨的积聚使细胞内UDP氨基己糖(UDP-N-乙酰葡糖胺和UDP-N-乙酰半乳糖胺)增加，影响细胞的生长及蛋白的糖基化过程。氨抑制Gln代谢途径，使Asp和Glu消耗增加。细胞消耗Asp增加，可能是细胞线粒体膜上苹果酸－天冬氨酸泵转运NADH加快，使细胞维持糖酵解途径的需要。Asp消耗增加可能会从Gln代谢多经天冬氨酸转氨酶途径而不是丙氨酸转氨酶或谷氨酸脱氢酶途径得以补偿
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