Merck蛋白抽提产品

【简单介绍】

BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法（如弗式压榨法和超声波法）的替代方法，这种试剂处理起来非常简便、快速，且成本低廉，适合制备表达蛋白用于下游的纯化等应用。

【详细说明】

Merck蛋白抽提

MERCK蛋白抽提

1、BugBuster®蛋白抽提试剂——尤其适用于GST融合蛋白的抽提BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法（如弗式压榨法和超声波法）的替代方法，这种试剂处理起来非常简便、快速，且成本低廉，适合制备表达蛋白用于下游的纯化等应用。 2、BugBuster Master Mix预混蛋白抽提试剂BugBuster Master Mix是将BugBuster蛋白抽提试剂，Benzonase核酸酶和rLysozyme™溶菌酶溶液按最优化配比混合好的一种非常方便使用的蛋白抽提试剂，有助于在最优化条件下获得可溶活性蛋白。

Product	Size	Cat.No.	Price（￥）
BugBuster® Protein Extraction Reagent	100 ml	70584-3	750.00
	500 ml	70584-4	3,789.00
BugBuster® Master Mix	100 ml	71456-3	1,700.00
	500 ml	71456-4	5,200.00
CytoBuster™ Protein Extraction Reagent	50 ml	71009-3	824.00
	250 ml	71009-4	3,122.00

Comparison of E. coli lysis methods50 ml samples of an induced 500 ml culture of BL21（DE3） containing pET-41a（+） encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity.

3、Benzonase® 核酸酶——赛多利斯病毒纯化试剂盒推荐使用应用范围

• ² 蛋白抽提操作中降低样品粘度
• ² 双向电泳中样品制备过程
• ² 重组蛋白样品制备中去除核酸污染

Product	Size	Cat.No.	Price（￥）
Benzonase® Nuclease, Purity > 90%	10 ku	70746-3	1,200.00
	2.5 ku	70746-4	400.00

Viscosity reduction by Benzonase NucleaseE. coli BL21（DE3） cells containing a pET construct were suspended in BugBuster Reagent （5 ml/g wet weight）. Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at

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