Comparison of E. colilysis methods50 ml samples of an induced 500 ml culture of BL21(DE3) containing pET-41a(+) encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity.
3、Benzonase® 核酸酶——赛多利斯病毒纯化试剂盒推荐使用应用范围
² 蛋白抽提操作中降低样品粘度
² 双向电泳中样品制备过程
² 重组蛋白样品制备中去除核酸污染
Product
Size
Cat.No.
Price(¥)
Benzonase® Nuclease, Purity > 90%
10 ku
70746-3
1,200.00
2.5 ku
70746-4
400.00
Viscosity reduction by Benzonase NucleaseE. coli BL21(DE3) cells containing a pET construct were suspended in BugBuster Reagent (5 ml/g wet weight). Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at