DL-二硫苏糖醇

产品名称
Application

用于将 −SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 。通常，使用 DTT (100mM) 所需的浓度是使用 (5% v/v, 700mM) 的浓度的七分之一。
Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.1
性质
Related Categories Alphabetical, BioUltra, Disulfide Bridge Cleavage Reagents, Monosaccharide, 分子生物学,
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grade for molecular biology
product line BioUltra
assay ≥99.5% (RT)
impurities DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.3% 4,5-dihydroxy-1,2-dithiane
pH 4.0-6.5 (25 °C, 0.1 M in H2O)
mp 41-44 °C(lit.)
solubility H2O: soluble0.1 M at 20 °C, clear, colorless
anion traces sulfate (SO42-): ≤50 mg/kg
cation traces Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤100 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
λ 0.1 M in H2O
UV absorption λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100
storage temp. 2-8°C