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- 英文名:
miaolingbio
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8888
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淼灵生物
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文献和实验Transduction Protocol for Adherent Cell Types
- The starting number of cells may be varied to account for total cell availability. Successful transductions have been carried with as little as 2.0 x 105 cells per well. - The duration the viral supernatant is on the cells can go from 4 hours to 2 days
实验试剂 人类胚胎肾(HEK)293T细胞 腺病毒转移载体质粒 腺病毒包装载体PLP1,pLP2(之前),PLP / VSVG DMEM培养基 青霉素,链霉素-谷氨酰胺(100X) 磷酸盐缓冲液PBS(-) 胎牛血清 凝聚胺 实验设备 10-cm的细胞培养皿 50毫升锥形离心管
The COS-7 Cell In Vitro Paradigm to Study Myelin Proteolipid Protein 1 Gene Mutations
of proteolipid protein 1 (PLP1) in transiently transfected COS-7 cells. In humans, the PLP1 gene is located on the long arm of the X-chromosome and deletion, duplication, or coding region mutations in this gene cause the leukodystrophy, Pelizaeus-Merzbacher
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