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- mol-innov
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- 2025年12月11日
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
Ig. In addition, young laboratory mice raised in sterile, pathogen free barriers and unimmunized have lower endogenous immunoglobulin levels than older animals raised in conventional housing. Most mouse monoclonal antibodies working
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