- 询价
- Cell Signaling Technology
- 9528
- USA
- 2025年10月30日
- W
- Rabbit
- H
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-cdc25C (Ser216) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
H
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 60 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-cdc25C (Ser216) Antibody detects endogenous levels of cdc25C only when phosphorylated at Ser216. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of wild type GST-cdc25C and mutant GST-cdc25C Ser216Ala fusion proteins, untreated or phosphorylated by Chk2, using Phospho-cdc25C (Ser216) Antibody. Western Blotting
Western blot analysis of GST-cdc25C phosphorylated by cdc2/cyclin B (New England Biolabs #P6020), Chk2 or both kinases, using Phospho-cdc25C (Ser216) Antibody (upper) or Phospho-cdc25C (Ser214) antibody (lower). Western Blotting
Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit mAb, #4688, (lower). |
| Background | cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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