- 询价
- Cell Signaling Technology
- 5468
- USA
- 2026年05月28日
- W, IP
- Rabbit
- H,M,R
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- 文献和实验
- 技术资料
- 抗体英文名：
BSP II Antibody
- 抗原：
synthetic peptide corresponding to residues near the amino terminus of human BSP II protein
- 应用范围：
W, IP
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 82 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | The BSP II Antibody detects endogenous levels of total BSP II protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BSP II protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from MDA-MB-435, U-2 OS, and Saos-2 cells using BSP II Antibody. |
| Background | Bone sialoprotein II (BSP II) exists as a secreted protein in extracellular matrix of the skeleton (1-2). BSP II is part of the small integrin binding ligand N-linked glycoprotein (SIBLING) family and its role is crucial for cell to matrix adhesion as well as between two active protein complexes (3). Flexible binding capacity of BSP II also allows it to rapidly associate with various proteins, calcium, and mineralized phases of developing bone (3-5). BSP II plays a central role in the initiation of hydroxyapatite microcalcification of breast cancer cells that contributes to the pathogenesis of bone metastasis (4,6,7). Recent evidence suggests that the downregulation of BSP II may lead to the reduced formation of metastatic lesions and bone metastasis in a human breast cancer model (5).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
antibody (mAb). Positive events (MHC class II/peptide complexes) are normalized and available for pattern analysis.
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 MHC II(MHC II)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 MHC II 单抗包被于酶标板上，标准品和样品中的 MHC II 与单抗结合，加入生物素化的抗大鼠 MHC II ，形成免疫复合
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