RCC2 Antibody

RCC2 Antibody

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年12月17日
  • W, IF-IC
  • Rabbit
  • H,M,R,Mk
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    • 详细信息
    • 技术资料
    • 抗体英文名

      RCC2 Antibody

    • 抗原

      synthetic peptide corresponding to residues near the carboxy terminus of human RCC2

    • 应用范围

      W, IF-IC

    • 宿主

      Rabbit

    • 级别

      详见MSDS文件

    • 保质期

      详见说明书

    • 供应商

      CST

    • 库存

      大量

    • 适应物种

      H,M,R,Mk

    • 是否单克隆

      2

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Source
    W IF-IC H M R Mk Endogenous 60 Rabbit
    Protocols

    * Product-specific protocol.

    Specificity / Sensitivity

    RCC2 Antibody detects endogenous levels of total RCC2 protein.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RCC2.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from HeLa, COS and C2C12 cells using RCC2 Antibody.

    IF-IC

    IF-IC

    Confocal immunofluorescent analysis of HeLa cells using RCC2 Antibody (green). Actin filaments have been labeled with Pan-Keratin (C11) Mouse mAb #4545 (red).

    Background

    RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC (reviewed in 1,2). In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B (3). RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres (4).

    1. Vader, G. et al. (2006) J Cell Biol 173, 833-7.
    2. Ruchaud, S. et al. (2007) Nat Rev Mol Cell Biol 8, 798-812.
    3. Mollinari, C. et al. (2003) Dev Cell 5, 295-307.
    4. Rosasco-Nitcher, S.E. et al. (2008) Science 319, 469-72.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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