EpCAM Antibody

3599:

Western Blotting

EpCAM Antibody detects endogenous levels of total EpCAM protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human EpCAM.

Western Blotting

Western blot analysis of extracts from HT-29, HCT-15 and HCT-116 cells using EpCAM Antibody.

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca²⁺ -independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).

1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
3. Armstrong, A. and Eck, S.L. Cancer Biol Ther 2, 320-6.

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• 2929 EpCAM (VU1D9) Mouse mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.