- 询价
- Cell Signaling Technology
- 9325
- USA
- 2025年10月18日
- W
- Rabbit
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
GSK-3β-Tag Antibody
- 抗原：
synthetic GSK-3β-Tag peptide
- 应用范围：
W
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
All
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | All | Endogenous | n/a | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GSK-3β-Tag Antibody detects transfected levels of GSK-3β-tagged proteins as well as endogenous GSK-3β (46 kDa). |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic GSK-3β-Tag peptide. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa cells, control (lane 1) or infected with YopE129-GSK-3β (lane 2 and 3), using GSK-3β-Tag Antibody (lysates kindly provided by Dr. Gregory Plano, University of Miami). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Short phosphorylatable peptide tags, such as the GSK-3β-tag, can be used to determine translocation of effector proteins in type III secretion systems (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验lizzy514 请问细胞中总的GSK-3β应该包括磷酸化的GSK-3β蛋白吧? 做WB检测细胞中GSK-3β和9位点磷酸化的GSK-3β(活性受抑制的形式)蛋白表达的变化,提取的是细胞总蛋白,那用GSK-3β抗体检测的GSK-3β的表达不仅仅只是非磷酸化的蛋白(活性形式)吧? 万分感谢!!! 坏坏的真心爱 有人回答吗？我也想知道 zhanglin1229 不是，GSK-3β
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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