Topoisomerase IIα Antibody

4733:

Flow , Immunoprecipitation , Western Blotting

Topoisomerase IIalpha Antibody detects endogenous levels of total Topoisomerase IIalpha protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Topoisomerase IIalpha. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from MDA-MB-468, MDA-MB-231 and HeLa cells, using Topoisomerase IIalpha Antibody.

Flow Cytometry

Flow cytometric analysis of untreated HeLa cells, using Topoisomerase IIalpha Antibody (blue) compared to a nonspecific negative control antibody (red).

DNA topoisomerase I and II are nuclear enzymes, and type II consists of two highly homologous isoforms: topoisomerase IIalpha and IIbeta. These enzymes regulate the topology of DNA, maintain genomic integrity and are essential for processes such as DNA replication, recombination, transcription and chromosome segregation by allowing DNA strands to pass through each other (1). Topoisomerase I nicks and rejoins one strand of the duplex DNA, but topoisomerase II transiently breaks and closes double-stranded DNA (2). Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g. etoposide) also convert topoisomerases to nucleases, and the enzyme is usually trapped as an intermediate, covalently bound to the 5+ end of the cleaved DNA strand(s). This intermediate leads again to genomic instability and cell death, and thus these agents that target topoisomerases are highly sought after cancer chemotherapeutic drugs (3). Ca2+ regulated phosphorylation of topoisomerase IIa Ser1106 modulates the activity of this enzyme as well as its sensitivity to targeting drugs (4).

1. Wang, J.C. (2002) Nat. Rev. Mol. Cell. Biol. 3, 430-440.
2. Pulleyblank, .E. (1997) Science 277, 648-649.
3. Li, T.K. and Liu, L.F. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 53-77.
4. Chikamori, K. et al. (2003) J. Biol. Chem. 278, 12696-12702.

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For Research Use Only. Not For Use In Diagnostic Procedures.