Phospho-LATS1 (Thr1079) (D57D3

8654:

Western Blotting

Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb detects endogenous levels of LATS1 protein only when phosphorylated at Thr1079. This antibody is predicted to cross react with LATS2 only when LATS2 is phosphorylated at Thr1041.

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr1079 of human LATS1 protein.

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or okadaic acid-treated (+), using Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb (upper) or LATS1 (C66B5) Rabbit mAb #3477 (lower).

LATS1 (Large tumor suppressor 1) is a putative serine/threonine kinase that belongs to the NDR family (1). It is a tumor suppressor that plays a critical role in the maintenance of ploidy. LATS1 localizes to the centrosome and the mitotic spindle and controls G2/M transition by negatively regulating cdc2 kinase activity (2,3). LATS1 also plays a role in the G1 tetraploidy checkpoint via control of p53 expression (4).LATS1 affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1 (5). LATS1 also binds the phosphorylated form of zyxin, a regulator of actin filament assembly. This interaction promotes localization of zyxin to the mitotic spindle, suggesting a role for actin regulatory proteins during mitosis (6). Decreased expression is associated with breast tumor aggressiveness (7), promoter methylation, and loss of heterozygosity. Mutations perturbing LATS1 have been associated with human sarcomas and ovarian sarcomas (8,9). LATS1 knock out mice develop soft-tissue sarcomas, ovarian stromal cell tumor, and display a high sensitivity to carcinogenic treatments (10).Human LATS1 exists in a complex similar to the Drosophila Hippo/Salvador/Lats tumor suppressor network, a complex that regulates proliferation and apoptosis to control growth and shape of the fly. The corresponding human complex contains Hippo and Salvador homologs RASSF1A, WW45, and MST2 and may control mitotic exit (11).Many molecules have been discovered that are involved in the Hippo pathway, but mechanisms governing activation of these proteins and activation of the pathway in general are currently not clear. Phosphorylation of LATS1 and LATS2 is stimulated by association with Mob1, which is itself phosphorylated by Mst1/2 (12,13). Phosphorylation of LATS1/2 is required for activation of YAP, a key mediator of the Hippo signaling pathway (14).

1. Tao, W. et al. (1999) Nat Genet 21, 177-81.
2. Yang, X. et al. (2001) Oncogene 20, 6516-23.
3. Xia, H. et al. (2002) Oncogene 21, 1233-41.
4. Iida, S. et al. (2004) Oncogene 23, 5266-74.
5. Yang, X. et al. (2004) Nat Cell Biol 6, 609-17.
6. Hirota, T. et al. (2000) J Cell Biol 149, 1073-86.
7. Morinaga, N. et al. (2000) Int J Oncol 17, 1125-9.
8. Hansen, L.L. et al. (2002) Cancer Genet Cytogenet 139, 1-8.
9. Hisaoka, M. et al. (2002) Lab Invest 82, 1427-35.
10. St John, M.A. et al. (1999) Nat Genet 21, 182-6.
11. Guo, C. et al. (2007) Curr Biol 17, 700-5.
12. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
13. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.
14. Zhao, B. et al. (2010) J Cell Sci 123, 4001-6.

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• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 4912 YAP Antibody
• 4911 Phospho-YAP (Ser127) Antibody
• 8418 YAP/TAZ (D24E4) Rabbit mAb
• 3863 MOB1 Antibody
• 2149 TAZ Antibody
• 5888 LATS2 (D83D6) Rabbit mAb
• 3682 Mst1 Antibody
• 3952 Mst2 Antibody
• 3507 Sav1 Antibody

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