Applications Key: W=Western Blotting F=Flow Cytometry Reactivity Key: H=Human Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
CD44 (156-3C11) Mouse mAb (Biotinylated) detects endogenous levels of total CD44 protein.
Source / Purification
Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.
Western Blotting
Western blot analysis of extracts from HeLa and PANC-1 cells using CD44 (156-3C11) Mouse mAb (Biotinylated) and developed using Streptavidin-HRP #3999.
Flow Cytometry
Flow cytometric analysis of HeLa cells using CD44 (156-3C11) Mouse mAb (Biotinylated) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097 (red).
Description
This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody exhibits the same species cross-reactivity as the unconjugated CD44 (156-3C11) Mouse mAb #3570.
Background
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).