β-Actin (13E5) Rabbit mAb (HRP

Conjugate)

价格询价

品牌Cell Signaling Technology
货号
5125
产地USA
更新日期2026年05月28日

应用范围W
适应物种H,M,R,Mk,B,Pg,C,Hr

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
β-Actin (13E5) Rabbit mAb (HRP Conjugate)
抗原：
synthetic peptide corresponding residues near the amino terminus of human β-actin
应用范围：
W
供应商：
CST
级别：
详见MSDS文件
保质期：
详见说明书
库存：
大量
适应物种：
H,M,R,Mk,B,Pg,C,Hr
标记物：
HRP
是否单克隆：
1
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W	H M R Mk B Pg (C) (Hr)	Endogenous	45	Rabbit IgG

Protocols	5125: Western Blotting
Specificity / Sensitivity	β-Actin (13E5) Rabbit mAb (HRP Conjugate) detects endogenous levels of total β-actin protein. This antibody may cross-react with γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth muscle, or γ-enteric smooth muscle isoforms.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding residues near the amino terminus of human β-actin. Western Blotting Western blot analysis of extracts from various cell lines using β-Actin (13E5) Rabbit mAb (HRP Conjugate).
Description	This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (β-Actin (13E5) Rabbit mAb #4970).
Background	Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6). Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.
Application References	Xiang, X. et al. (2011) PLoS One 6, e14640. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	4970 β-Actin (13E5) Rabbit mAb 3311 Phospho-Cofilin (Ser3) Antibody 3312 Cofilin Antibody 4967 β-Actin Antibody 1025 β-Actin Blocking Peptide 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7727 Biotinylated Protein Ladder Detection Pack 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7003 20X LumiGLO^® Reagent and 20X Peroxide 4968 Pan-Actin Antibody 9967 Actin Reorganization Antibody Sampler Kit 8112 SignalStain^® Antibody Diluent Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures.