- 询价
- Cell Signaling Technology
- 5125
- USA
- 2025年09月10日
- W
- H,M,R,Mk,B,Pg,C,Hr
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
β-Actin (13E5) Rabbit mAb (HRP Conjugate)
- 抗原：
synthetic peptide corresponding residues near the amino terminus of human β-actin
- 应用范围：
W
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
H,M,R,Mk,B,Pg,C,Hr
- 标记物：
HRP
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk B Pg (C) (Hr) | Endogenous | 45 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | β-Actin (13E5) Rabbit mAb (HRP Conjugate) detects endogenous levels of total β-actin protein. This antibody may cross-react with γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth muscle, or γ-enteric smooth muscle isoforms. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding residues near the amino terminus of human β-actin. Western Blotting
Western blot analysis of extracts from various cell lines using β-Actin (13E5) Rabbit mAb (HRP Conjugate). |
| Description | This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (β-Actin (13E5) Rabbit mAb #4970). |
| Background | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
according to my protocol. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP
家兔（Rabbit,Oryctolagus curiculus）
家兔（Rabbit,Oryctolagus curiculus） 一、生物学特性和解剖生理特点 1．家兔属于哺乳纲、啮齿目、兔科、草食性哺乳动性。 2．家兔体小力弱、胆小怕惊、怕热、怕潮，喜欢安静、清洁、干燥、凉爽的环境，不能忍受污秽的条件。 3．是食草类（Herbivorous）单胃动物。饲养原则是以青粗食米为主，精饲料为辅。喜欢独居，白天活动少，都处于假眠或休息状态，夜间活动大，吃食多。有啃木、扒土的习惯
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