- 询价
- R&D
- 美国/中国
- xy-532R
- 2025年07月11日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Beta Amyloid (Aβ) Monoclonal Antibody保存于运输说明：
- Store at -20°C.
- 详细信息：
-
Description 
Monoclonal Antibody against Amyloid beta (Aβ) Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone NAB 228 Form Ascites Host Beta Amyloid (Aβ) Monoclonal AntibodyMouse Species Reactivity Human IsoType IgG2a [Ab] 2-3 mg/mL Specificity Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APPs) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse species of Aβ with differential propensities to fibrillize and deposit in Senile Plaques. Amyloid beta (Aβ) NAB228 antibody recognizes the Amyloid beta (Aβ) 1-11 of the human beta amyloid peptide as determined by ELISA using peptides to block immunoreactivity. NAB228 also recognizes full length APP, sAPP species excluding sAPPβ, C99 and Aβ. Uses Beta Amyloid (Aβ) Monoclonal AntibodyThis antibody is effective in immunoblotting (WB), immunoprecipitation (IP), immunohistochemistry (IHC), ELISA and sandwich ELISA. Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - WB: 1:500
- IHC: 1:10,000-100,000*
- IP: 1:500
- ELISA: 10 ng/mL
Notes *This antibody can be used to stain amyloid plaques (diffuse and compact) and amyloid angiopathy on both ethanol or formalin fixed paraffin embedded tissue (human AD, Downs syndrome, MCI and transgenic mouse). Formic acid pretreatment is not necessary although signal is improved if it is used. Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Lee EB, Skovronsky DM, Abtahian F, Doms RW, Lee VM. Secretion and intracellular generation of truncated Abeta in beta-site amyloid-beta precursor protein-cleaving enzyme expressing human neurons. J Biol Chem 278(7):4458-4466, 2003. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Beta Amyloid (Aβ) Monoclonal Antibody
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文献和实验Evaluating Amyloid Beta (A) 1-40 Degradation by Capillary Electrophoresis
Here, we describe a capillary electrophoresis method for evaluating proteolysis of the amyloid beta peptide (Aβ) 1–40. This method is suitable for kinetic studies, demands little specialized equipment, and consumes only small
Detecting Amyloid- Plaques in Alzheimers Disease
One of the major neuropathological changes characteristic of Alzheimer’s disease (AD) is deposits of beta-amyloid plaques and neurofibrillary tangles in neocortical and subcortical regions of the AD brain. The histochemical detection
Western Blotting with Monoclonal Ant
). 2.Aspirate excess PBS. 3.Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol). 4.Scrape cells from dish, transfer to a microcentrifuge tube, and boil
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