- 询价
- R&D
- 美国/中国
- xy-627R
- 2025年07月01日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Neuronal Nicotinic Acetylcholine Receptor, alpha7-Subunit, Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody against Neuronal Nicotinic Acetylcholine Receptor, alpha7-Subunit Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone 306 Form Ascites Host Mouse Species Reactivity Neuronal Nicotinic Acetylcholine Receptor, alpha7-Subunit, Monoclonal AntibodyHuman, Rat, Chicken IsoType IgG1 [Ab] 1-2 mg/mL Specificity This antibody was generated in mice against purified rat and chicken brain alpha-7 neuronal nicotinic acetylcholine receptors (nAChRs). It binds to the cytoplasmic surface of chicken alpha-7 AChRs between amino acids 380-400. Clone 306 binds to both native and denatured chicken alpha-7. It also binds to denatured rat and human alpha-7 but with lower affinity than to chicken alpha-7. This antibody is also useful histologically. Uses This antibody is effective in immunoblotting, immunofluorescence (IF), immunohistochemistry (IHC) and immunoprecipitation (IP). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:1,000*
- IF: 1:500
- IHC: 1:100
- IP: 1:150
Notes Neuronal Nicotinic Acetylcholine Receptor, alpha7-Subunit, Monoclonal AntibodyPredicted MW = 57 kDa Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Dominguez del Toro E, Juiz JM, Peng X, Lindstrom J, Criado M. Immunocytochemical localization of the alpha 7 subunit of the nicotinic acetylcholine receptor in the rat central nervous system. J Comp Neurol 349:325-342, 1994. [Wb, IHC, IP]
Peng X, Katz M, Gerzanich V, Anand R, Lindstrom J. Human alpha 7 acetylcholine receptor: cloning of the alpha 7 subunit from the SH-SY5Y cell line and determination of pharmacological properties of native receptors and functional alpha 7 homomers expressed in Xenopus oocytes. Mol Pharmacol 45:546-554, 1994.
McLane KE, Wu X, Lindstrom JM, Conti-Tronconi BM. Epitope mapping of polyclonal and monoclonal antibodies against two alpha-bungarotoxin-binding alpha subunits from neuronal nicotinic receptors. J Neuroimmunol 38:115-128, 1992.
Schoepfer R, Conroy WG, Whiting P, Gore M, Lindstrom JM. Brain alpha-bungarotoxin binding protein cDNAs and MAbs reveal subtypes of this branch of the ligand-gated ion channel gene superfamily. Neuron 5:35-48, 1990.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Neuronal Nicotinic Acetylcholine Receptor, alpha7-Subunit, Monoclonal Antibody
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文献和实验相关专题 细胞凋亡专题 烟酰胺乙酰胆碱受体在凋亡中的作用 Nicotinic acetylcholine receptors are essential for neuromuscular signaling and are also expressed in non-neuronal tissues, where their function is less clear. Although nicotinic
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Recombinant Cell Lines Stably Expressing Functional Ion Channels
acetylcholine receptor alpha7 (nAChRα7) in a host cell, we show a way to construct and use such a stable cell-based expression system for electrophysiological assays.
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