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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- β-Tubulin Monoclonal Antibody保存于运输说明:
- Store at -20°C
- 详细信息:
-
Description 
Monoclonal Antibody against &beta-Tubulin Clone 
2-28-33 Form 
Ascites Host 
Mouse Species Reactivity 
Bovine, Mouse, Rat IsoType 
β-Tubulin Monoclonal AntibodyIgG1 [Ab] 
2-3 mg/mL Specificity 
Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of α- and β-tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. Clone 2-28-33 has been widely used in research as a marker for beta tubulin and reacts with two major and one minor β-tubulin isotypes. This antibody was raised against Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. Uses 
β-Tubulin Monoclonal AntibodyThis antibody is effective in immunoblotting and immunostaining. Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000*
- Immunostaining: 1:1,000
Notes 
*Predicted MW = 50 kD.
Recommended positive control = NIH3T3 and HeLa cells.Storage 
Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw. References 
Siddiqui SS, Aamodt E, Rastinejad F, Culotti J. Anti-tubulin monoclonal antibodies that bind to specific neurons in Caenorhabditis elegans. J. Neurosci. 9(8):2963-72, 1989.
G Piperno, M LeDizet, XJ Chang. Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. J. Cell. Biol. 104(2):289-302, 1987.
M LeDizet, G Piperno. Cytoplasmic microtubules containing acetylated alpha-tubulin in Chlamydomonas reinhardtii: spatial arrangement and properties. J. Cell. Biol. 103(1):13-22, 1986.
G Piperno, MT Fuller. Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms. J. Cell. Biol. 101(6):2085-2094, 1985.Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - β-Tubulin Monoclonal Antibody
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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