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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- CD50 Monoclonal Antibody, Purified (SIGNET)详细信息:
-
Description 
Purified Monoclonal Antibody against CD50
(Formerly Signet Catalog No: 648-26 L2 Predilute)Intended Use 
**Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone 
186-2G9 Form 
Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host 
CD50 Monoclonal Antibody, Purified (SIGNET)Mouse Species Reactivity 
Human IsoType 
IgG2b Specificity 
The Signet CD50 (186-2G9) antibody reacts with the single chain 120kDa polypeptide cellular adhesion molecule (CD50 antigen ICAM-3).
This antibody was developed using stimulated human leukocytes.
Prediluted Format:
SIG-3648-26 [L2 6 mL] is ready-to-use with Biotin-based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).Uses 
This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution 
CD50 Monoclonal Antibody, Purified (SIGNET)The optimal working dilution should be determined for each specific assay condition. - IHC: Ready-to-use with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250)
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Sodium Citrate HIER (SIG-31932)
Incubation: 20 minutes at room temperature
Notes 
Positive tissue (human): Tonsil Storage 
Store between 2-8°C. References 
Terol MJ, et al. Expression of intracellular adhesion molecule-3 (ICAM-3/CD50) in malignant lymphoproliferative disorders and solid tumors. Tissue Antigens 48:271-7, 1996.
Juan M, et al. CD50 stimulation induces calcium mobilization and tyrosine phosphorylation through p59fyn and p56lck in jurkat t cell line. J Exp Med 179(6):1747-56, 1994.
Cid, MC, et al. Signaling through CD50 (ICAM 3) stimulates T lymphocytes binding to human umbilical vein endothelial cells and extracellular matrix proteins via an increase in beta 1 and beta 2 integrin function. Eur J Immunol 24(6):1377-82, 1994.Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - IHC: Ready-to-use with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250)
- CD50 Monoclonal Antibody, Purified (SIGNET)
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