- 询价
- R&D
- 美国/中国
- xy-3414
- 2025年07月11日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Immunoglobulin M (IgM) Polyclonal Antibody, Purified (SIGNET)详细信息：
-
Description 
Purified Polyclonal Antibody against Immunoglobulin M (IgM)
(Formerly Signet Catalog Nos: 414-01, 414-16 L1 Predilute, 414-26 L2 Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Form Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Rabbit Species Reactivity Immunoglobulin M (IgM) Polyclonal Antibody, Purified (SIGNET)Human, Baboon, Dog and Monkey Specificity The Signet anti-Immunoglobulin M (IgM) antibody recognizes the mu heavy chains of human IgM. This antibody was developed using human IgM.
Concentrated Format:
SIG-3414-1000 [1 mL]
Prediluted Formats:
SIG-3414-16 [6 mL]
SIG-3414-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).Uses Immunoglobulin M (IgM) Polyclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All™ 1 (SIG-31910).
Incubation: 20 minutes at room temperature
Notes Positive tissue (human): Tonsil Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
- Immunoglobulin M (IgM) Polyclonal Antibody, Purified (SIGNET)
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
with non-immune reactivity for immunoglobulin G. J. Immunol. 111:1401-1406. Richman, D. D., Cleveland, P. H., Oxman, M. N., and Johnson, K. M. 1982. The binding of staphylococcl protein A by the sera of different animal species. J. Immunol. 128
Introduction to the Purification of Antibodies
the production of monoclonal antibodies is more time consuming and requires tissue culture facilities and skills. The use of either monoclonal or polyclonal antibodies in some of the applications may require that the antibody is in a purified form
Here we describe a detailed protocol for the one-step preparation of antigen-specific human chimeric immunoglobulin G (IgG) monoclonal antibodies (mAbs) using an in vitro antibody design method referred to as the ADLib (Autonomously
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