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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
CD3 (CD3) (Cytoplasmic Domain) 抗体
CD3 (CD3) (Cytoplasmic Domain) 抗体
抗原
CD3 (CD3)
别名 CD3
抗原表位
其他选择 Cytoplasmic Domain
适用
其他选择 犬, 小鸡, 马, 人, 小鼠, 猪
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (CD3-12)
标记
其他选择 非结合性
CD3 (CD3) (Cytoplasmic Domain) 抗体应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术（FACS）, 免疫萤光（IF）, Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), 免疫组织化学石蜡技术（IHC-P）
Pubmed 10个引用可参考
规格 0.2 mg
发货至 中国 (更改)
产品细节
免疫原 Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
序列 Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys
克隆位点 CD3-12
亚型 IgG1
特异性 This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).
交叉反应 （详细） Species reactivity (expected):Mammals, Birds.
Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena.
CD3 (CD3) (Cytoplasmic Domain) 抗体纯化方法 Affinity Chromatography on Protein G
目标详细情况
别名 CD3
背景 T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
基因ID 916
NCBI登录号 NP_000724
UniProt P07766
研究领域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
使用细节
应用备注 Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
浓度 1.0 mg/mL
缓冲液 PBS, 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store the reconstituted antibody undiluted at 2-8 °C.
CD3 (CD3) (Cytoplasmic Domain) 抗体相关产品如下：xy-9015R EFHD1EFHD1蛋白抗体
xy-9016R EF-CBP2突触结合蛋白2抗体
xy-6279R ENPP2核苷酸焦磷酸酶2抗体
xy-2920R ECHS1长链烯脂酰辅酶A水化酶抗体
xy-6273R ENO1+ENO2+ENO3神经元，肌肉特异性烯醇化酶抗体
xy-7541R EDG3内皮分化型G蛋白偶联受体3抗体
xy-6380R ERCC6L发育相关蛋白ERCC6L抗体（乙醇致畸因子）
xy-4920R Escherichka coli K99猪源产肠毒素性大肠杆菌K99抗体
xy-11793R ENSAα内磺肽抗体
xy-10012R phospho-E2F1 (Ser337)磷酸化转录因子E2F-1抗体
xy-7058R EMR1表皮生长因子样激素受体1抗体
xy-6954R Ep300CREB结合蛋白p300抗体
xy-4700R LMP2AEB病毒LMP-2A蛋白抗体
xy-6938R EBV Nuclear AntigenEB病毒核抗原1抗体
xy-6959R phospho-Estrogen Receptor beta (Ser87)磷酸化雌激素受体β抗体
xy-6420R phospho-eIF4EBP1 (Thr36)磷酸化4E结合蛋白1抗体
xy-6421R phospho-eIF4EBP1+2+3 (Thr45)磷酸化4E结合蛋白1,2,3抗体
xy-9485R Epsin2BEpsin2B蛋白抗体
xy-4567R EGF表皮生长因子抗体
xy-9661R EGFL8表皮生长因子样蛋白8抗体
xy-9663R E3 ubiquitin ligaseE3泛素连接酶抗体
xy-4568M EGF小鼠抗表皮生长因子抗体
xy-4568R EGF抗表皮生长因子抗体
xy-7553R Eph receptor A2+A3+A4内皮细胞受体蛋白酪氨酸激酶A2+A3+A4抗体
xy-7554R phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596)磷酸化内皮细胞受体蛋白酪氨酸激酶A2+A3+A4抗体
xy-7555R phospho-EPH A3+A4+A5 ( Tyr779)磷酸化内皮细胞受体蛋白酪氨酸激酶A3+A4+A5抗体
xy-8592R Etv5转录因子Ets差异基因5抗体
xy-1273R E-SelectinE选择素抗体
xy-8858R heatstable enterotoxin 1 [Escherichia coli]大肠杆菌耐热性肠毒素抗体
xy-6835R E1 Ubiquitin Activating Enzyme泛素激活酶E1抗体
xy-10007R EGFR表皮生长因子受体抗体
xy-1285R ETS1Ets转录因子家族ets1抗体
抗原
CD3 (CD3)
别名 CD3
抗原表位
其他选择 Cytoplasmic Domain
适用
其他选择 犬, 小鸡, 马, 人, 小鼠, 猪
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (CD3-12)
标记
其他选择 非结合性
CD3 (CD3) (Cytoplasmic Domain) 抗体应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术（FACS）, 免疫萤光（IF）, Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), 免疫组织化学石蜡技术（IHC-P）
Pubmed 10个引用可参考
规格 0.2 mg
发货至 中国 (更改)
产品细节
免疫原 Synthetic peptide sequence derived from Cytoplasmic epitope of CD3
序列 Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys
克隆位点 CD3-12
亚型 IgG1
特异性 This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species cross reactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005).
交叉反应 （详细） Species reactivity (expected):Mammals, Birds.
Species reactivity (tested):Human, Mouse, Canine, Chicken, Equine, Porcine and Rhesus Monkey, Alpaca, Amazon Parrot, Duck, Harbour Porpoise, Koala, Raccoon, Sea Lion, Spotted Hyena.
CD3 (CD3) (Cytoplasmic Domain) 抗体纯化方法 Affinity Chromatography on Protein G
目标详细情况
别名 CD3
背景 T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
基因ID 916
NCBI登录号 NP_000724
UniProt P07766
研究领域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
使用细节
应用备注 Flow cytometry (1/50-1/100, requires membrane permeabilisation). Immunohistochemistry on frozen sections and paraffin embedded tissues (1/1000). This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1 mM EDTA pH 8.0 is recommended for this purpose. Recommended positive control tissue: Tonsil. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
浓度 1.0 mg/mL
缓冲液 PBS, 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store the reconstituted antibody undiluted at 2-8 °C.
CD3 (CD3) (Cytoplasmic Domain) 抗体相关产品如下：xy-9015R EFHD1EFHD1蛋白抗体
xy-9016R EF-CBP2突触结合蛋白2抗体
xy-6279R ENPP2核苷酸焦磷酸酶2抗体
xy-2920R ECHS1长链烯脂酰辅酶A水化酶抗体
xy-6273R ENO1+ENO2+ENO3神经元，肌肉特异性烯醇化酶抗体
xy-7541R EDG3内皮分化型G蛋白偶联受体3抗体
xy-6380R ERCC6L发育相关蛋白ERCC6L抗体（乙醇致畸因子）
xy-4920R Escherichka coli K99猪源产肠毒素性大肠杆菌K99抗体
xy-11793R ENSAα内磺肽抗体
xy-10012R phospho-E2F1 (Ser337)磷酸化转录因子E2F-1抗体
xy-7058R EMR1表皮生长因子样激素受体1抗体
xy-6954R Ep300CREB结合蛋白p300抗体
xy-4700R LMP2AEB病毒LMP-2A蛋白抗体
xy-6938R EBV Nuclear AntigenEB病毒核抗原1抗体
xy-6959R phospho-Estrogen Receptor beta (Ser87)磷酸化雌激素受体β抗体
xy-6420R phospho-eIF4EBP1 (Thr36)磷酸化4E结合蛋白1抗体
xy-6421R phospho-eIF4EBP1+2+3 (Thr45)磷酸化4E结合蛋白1,2,3抗体
xy-9485R Epsin2BEpsin2B蛋白抗体
xy-4567R EGF表皮生长因子抗体
xy-9661R EGFL8表皮生长因子样蛋白8抗体
xy-9663R E3 ubiquitin ligaseE3泛素连接酶抗体
xy-4568M EGF小鼠抗表皮生长因子抗体
xy-4568R EGF抗表皮生长因子抗体
xy-7553R Eph receptor A2+A3+A4内皮细胞受体蛋白酪氨酸激酶A2+A3+A4抗体
xy-7554R phospho-Eph receptor A2+A3+A4 (Tyr588 + Tyr596)磷酸化内皮细胞受体蛋白酪氨酸激酶A2+A3+A4抗体
xy-7555R phospho-EPH A3+A4+A5 ( Tyr779)磷酸化内皮细胞受体蛋白酪氨酸激酶A3+A4+A5抗体
xy-8592R Etv5转录因子Ets差异基因5抗体
xy-1273R E-SelectinE选择素抗体
xy-8858R heatstable enterotoxin 1 [Escherichia coli]大肠杆菌耐热性肠毒素抗体
xy-6835R E1 Ubiquitin Activating Enzyme泛素激活酶E1抗体
xy-10007R EGFR表皮生长因子受体抗体
xy-1285R ETS1Ets转录因子家族ets1抗体
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文献和实验相关实验
- 3) 及 CD45 -PE (HI30，白细胞标志) 抗体染色后，进行流式分析，死细胞以 DAPI 染色先行排除，左右分别为分选前后的细胞群分布。 总结： 几次实验的结果，使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选，平均得率为 84.5%，纯度大于 90%，细胞存活率高于 95%，且目标细胞不含标记，贴近天然，使下游实验更精确。
CD3 分子 CD3 常用单克隆抗体或代号：T3，I．eu4，HCHTl 主要表达细胞：T，Thy T细胞 分子质量(kDa)和结构：γ，δ，ε，ζ，η分别为26，20， 19， 16， 2l 功 能：TCR／CD3复合体．T细胞信号转导 CD3 Aka OKT3 Function: complex (5 chains) of integral membrane
及释放目标细胞。以不具磁性且可逆的试剂，取代一般具高亲和力的完整抗体，从而达到直接由全血或其他样本，进行温和标准化正选的目的。Fab-TACS 正选，化解了传统使用完整抗体进行正选的困境，其对目标细胞无不必要的刺激，避免了分选流程的细胞活化，也避免完整抗体与特定受体的结合而影响的分析准确度。因此，使用 Fab-TACS 无痕正选技术可富集不含标记、未激活的目标细胞，次次精准。人全血 CD3+ T 细胞分选实例1. 所需产品：(1) CD3 Fab-TACS® Gravity Kit, human
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CD3 (CD3) (Cytoplasmic Domain) 抗体
询价
