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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- Immunoglobulin G (IgG) Polyclonal Antibody, Purified (SIGNET)详细信息:
-
Description 
Purified Polyclonal Antibody against Immunoglobulin G (IgG)
(Formerly Signet Catalog Nos: 411-01, 411-16 L1 Predilute, 411-26 L2 Predilute)Intended Use 
** In Vitro Diagnostic (IVD) ** Form 
Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host 
Rabbit Species Reactivity 
Immunoglobulin G (IgG) Polyclonal Antibody, Purified (SIGNET)Human Specificity 
The Signet anti-Immunoglobulin G (IgG) antibody recognizes the γ heavy chains of human IgG. This antibody was developed using human IgG.
Concentrated Format:
SIG-3411-1000 [1 mL]
Prediluted Formats:
SIG-3411-16 [6 mL]
SIG-3411-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).Uses 
Immunoglobulin G (IgG) Polyclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (SIG-31910).
Incubation: 20 minutes at room temperature
Notes 
Positive tissue (human): Tonsil Storage 
Store between 2-8°C. Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
- Immunoglobulin G (IgG) Polyclonal Antibody, Purified (SIGNET)
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文献和实验Purification of Immunoglobulin G (IgG)
immunoglobulin, and some procedures may yield artifactual results if whole antiserum or ascitic fluid is used as a source of antibody. Purification of immunoglobulins is therefore essential or, at least, useful for a range of immunological methods. This process
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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