- 询价
- R&D
- 美国/中国
- xy-32612
- 2025年07月15日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 700 Tests (SIGNET)详细信息：
-
Description 
ACUITY Biotin Free Polymer Detection HRP System (Cap Gap) Complete, 700 Tests
(Formerly Signet Catalog No.: 2612)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Detection Kit Specificity ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 700 Tests (SIGNET)Signets ACUITY Detection system is a sensitive biotin-free polymer detection kit that is compatible with antibodies raised in mouse, rat and rabbit. ACUITY contains a horseradish Peroxidase labeled polymer and special blocking solutions to eliminate possible nonspecific background staining.
Kit Contains:- Serum Block, 125 mL
- Special Block, 125 mL
- HRP Polymer Link, 125 mL
- DAB Substrate Buffer, 2x125 mL
- DAB Chromogen, 6 mL
- Hydrogen Peroxide, 125 mL
Suggested Working Dilution
Protocol:
1. Tissue preparation: Deparaffinize and rehydrate according to the established protocols.
2. Blocking step-1: Incubate with H202 at RT for 15 minutes. Rinse with PBS.
3. Blocking step-2: Apply the Serum Block reagent for 15 minutes.
4. Primary antibody: Apply the primary antibody for 30 minutes.
5. Blocking step-3: Apply the Special Block reagent for 20 minutes then rinse with PBS (5min/2X).
ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 700 Tests (SIGNET)6. Polymer Link: Apply the Polymer reagent for 30 minutes then rinse with PBS (5min/2X).
7. Chromogen step: Apply DAB for 3-5 minutes then rinse with DI water.
8. Counter stain & mount.Notes Discard unused DAB working solution at the end of the run. Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - ACUITY Biotin Free Polymer Detection HRP (Cap Gap) Complete 700 Tests (SIGNET)
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文献和实验BioID: A Screen for Protein‐Protein Interactions
for each experimental condition (cells expressing BioID constructs or control cells) Complete medium 1 mM (20×) biotin (see recipe
,000) prior to visualization with Immobilon Western HRP Chemiluminescent Substrate. The blots were then stripped by (B) low pH or (C) heat/detergent and re-exposed to the secondary antibody and the Immobilon Western substrate to demonsrate complete
Studies of the Ubiquitin Proteasome System
Biomedicals Life Science Division, or see recipe ) or HRP‐conjugated anti–rabbit Ig and chemiluminescent detection reagents (SuperSignal West Pico, Pierce, or ECL Plus, GE Healthcare) Biomax MR film (Kodak
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