- 询价
- R&D
- 美国/中国
- xy-32606
- 2025年07月12日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- ACUITY Biotin Free Polymer Detection HRP 500 Tests (SIGNET)详细信息：
-
Description 
ACUITY Biotin Free Polymer Detection HRP System, 500 Tests
(Formerly Signet Catalog No.: 2606)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Detection Kit Specificity Signets ACUITY Detection system is a sensitive biotin-free polymer detection kit that is compatible with antibodies raised in mouse, rat and rabbit. ACUITY contains a horseradish Peroxidase labeled polymer and special blocking solutions to eliminate possible nonspecific background staining.
ACUITY Biotin Free Polymer Detection HRP 500 Tests (SIGNET)Kit Contains:- Serum Block, 50 mL
- Special Block, 50 mL
- HRP Polymer Link, 50 mL
Suggested Working Dilution
Protocol:
1. Tissue preparation: Deparaffinize and rehydrate according to the established protocols.
2. Blocking step-1: Incubate with H202 at RT for 15 minutes. Rinse with PBS.
3. Blocking step-2: Apply the Serum Block reagent for 15 minutes.
4. Primary antibody: Apply the primary antibody for 30 minutes.
5. Blocking step-3: Apply the Special Block reagent for 20 minutes. then rinse with PBS (5min/2X).
6. Polymer Link: Apply the Polymer reagent for 30 minutes then rinse with PBS (5min/2X).
7. Chromogen step: Apply DAB or AEC for 3-5 minutes then rinse with DI water.
ACUITY Biotin Free Polymer Detection HRP 500 Tests (SIGNET)8. Counter stain & mount.Notes Discard unused DAB or AEC working solution at the end of the run. Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - ACUITY Biotin Free Polymer Detection HRP 500 Tests (SIGNET)
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文献和实验ImmunoResearch Laboratories Inc.; Catalog No. 200-032-156) or HRP-conjugated sheep monoclonal anti-DIG (1/500 dilution of stock solution in PBTB; Roche Applied Science; Catalog No. 1 207 733). 7. Detection of biotin-labeled probe: Streptavidin-HRP
Immunohistochemistry on fixed, paraffin-embedded sections
-conjugated avidin (50 to 100 µl, dilution 1:300 -500) (Dako P0364) and incubate for 20 min. Be careful not to dilute the avidin-HRP in biotin-containing or azide-containing medium. 10- Wash thrice in TBS-T. 11- add 50 ml of the developing
免疫组化技术-Immunohistochemical Protocols
. Wash in PBS 3x5 min Incubate with an HRP-conjugated (Table 2) or a biotin-conjugated (Table 2) secondary antibody, both at a dilution of 1:500 in BSA diluent for 1 hr
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