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- 美国/中国
- xy-3611
- 2025年07月16日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- GCDFP-15 Monoclonal Antibody, Purified (SIGNET)详细信息：
-
Description 
Monoclonal Antibody against BRST-1 (CU-18)
(Formerly Signet Catalog Nos: 610-01, 610-16 L1 Predilute, 610-26 L2 Predilute, 610-36 LV Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone CU-18 Form Tissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity Human IsoType GCDFP-15 Monoclonal Antibody, Purified (SIGNET)IgG1 Specificity The BRST-1, clone CU-18, monoclonal antibody detects BCA-225, a 225kDa glycoprotein secreted by the T47D breast carcinoma cell line. Strong intracytoplasminc staining is seen in primary and metastatic breast carcinoma cell lines, primary and metastatic breast carcinoma tissue and cervical carcinomas.
This antibody was generated against a protein secreted by the T47D (Clone 11) human breast carcinoma cell line.
Concentrated Format:
SIG-3610-1000 [1 mL]
Prediluted Formats:
SIG-3610-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems.
SIG-3610-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
SIG-3610-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES and Ventana NexES automated immunostainers.Uses This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20 minutes at room temperature
Notes GCDFP-15 Monoclonal Antibody, Purified (SIGNET)Positive tissue (human): Breast carcinoma Storage Store between 2-8°C. References Imam SA, et al. Comparison of two microwave based antigen retrieval solutions in unmasking epitopes in formalin-fixed tissue for immunostaining. Anticancer Res 15:1153-8, 1995.
Ansai S, et al. An immunohistochemical study of BCA-225 in various skin cancers. J Dermatol 21(1): 20-4, 1994.
Ma CK, et al. Comparative immunohistochemical study of primary and metastatic carcinomas of the liver. Am J Clin Pathol 99(5):551-7, 1993.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
- GCDFP-15 Monoclonal Antibody, Purified (SIGNET)
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文献和实验Monoclonal Antibody Affinity Chromatography
all the above criteria and also afford the advantage of being available in essentially unlimited supply. Moreover, they are themselves readily purified, often by affinity chromatography. The main disadvantage of monoclonal antibody ligands is that they must possess
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
proteins. Taking advantage of the exquisite specificity afforded by each antibody results in a final purified product that binds specifically to that MAb. Using immunoaffinity chromatography, a single antigen can be separated from very complex mixtures (e.g
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