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- 美国/中国
- xy-3622
- 2025年07月29日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Apo-D (GCDFP-24) Monoclonal Antibody, Purified (SIGNET)详细信息：
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Description 
Purified Monoclonal Antibody against Apolipoprotein-D (GCDFP-24)
(Formerly Signet Catalog No: 622-01)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone 8CD6 Form Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity Apo-D (GCDFP-24) Monoclonal Antibody, Purified (SIGNET)Human IsoType IgG1 Specificity The anti-APO-D antibody, clone 8CD6, recognizes Apolipoprotein-D, a 24 kDa progesterone binding glycoprotein.
This antibody was developed using APO-D protein.Uses This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 for Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (SIG-31910) or Sodium Citrate HIER (SIG-31932).
Incubation: 20-60 minutes at room temperature
Notes Positive tissue (human): Prostate carcinoma
Storage Apo-D (GCDFP-24) Monoclonal Antibody, Purified (SIGNET)Store between 2-8°C. References Aspinall JO, Bentel JM, Horsfall DJ, Haagensen DE, Marshall VR, Tilley WD. Differential expression of apolipoprotein-D and prostate specific antigen in benign and malignant prostate tissues. J Urol 154:622-628, 1995. (GI)
Mazoujian G, Haagensen D. The immunopathology of gross cystic disease fluid proteins. Ann NY Acad Sci 586:188-97, 1990.
Balbin M, Freije JM, Fueyo A, Sanchez LM, Lopez-Otin C. Apolipoprotein D is the major protein component in cyst fluid from women with human breast gross cystic disease. Biochem J 271(3):803-7, 1990.
Mazoujian G. Immunohistochemistry of GCDFP-24 and zinc alpha2 glycoprotein in benign sweat gland tumors. Am J Dermatopathol 12(5):452-7, 1990.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 for Biotin based detection systems such as Signets USA Ultrastreptavidin Detection (SIG-32250).
- Apo-D (GCDFP-24) Monoclonal Antibody, Purified (SIGNET)
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文献和实验Monoclonal Antibody Production
Positive Clones (1) The day prior to any expansion,obtain feeder cells.For this first expansion from the 200 µl cultures in 96-well plates to 500 µl cultures in 24-well plates,add 200 µl of feeder cells/well in DMEM/20% FCSHT to the 24-well plates the day
Monoclonal Antibody Affinity Chromatography
all the above criteria and also afford the advantage of being available in essentially unlimited supply. Moreover, they are themselves readily purified, often by affinity chromatography. The main disadvantage of monoclonal antibody ligands is that they must possess
Monoclonal Antibody Production Protocol
/ml). Add Aminopterin (2ml to 200 ml of medium). (Some workers will leave the cells at this point for 24 hours before adding Aminopterin. We add the drug immediately.) Seed the cells in 96 well microtiter dishes, 250 ul per well, 8 plates per fusion
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