- 询价
- R&D
- 美国/中国
- xy-26R
- 2025年07月12日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- GFAP (SMI 26) Monoclonal Antibody Cocktail详细信息：
-
Description 
Monoclonal antibody cocktail against glial fibrillary acidic protein (GFAP) derived from the three Bigner-Eng clones Mab1B4, MAb2E1 and Mab4A11 and clone SMI-21 Clone SMI-26 Form Ascites Fluid (contains 0.01M sodium azide) Host Mouse Species Reactivity human, sheep, cow, dog, pig, rat, guinea pig, rat, mouse and chicken IsoType GFAP (SMI 26) Monoclonal Antibody Cocktail IgG2b and IgG1 (SMI-21) Specificity The Bigner-Eng antibodies have originally been assayed by indirect radioimmunoassay against fixed cell monolayers of a GFAP-positive human glioma cell line and also by competitive radioimmunoassay with radiolabelled GFAP and by competitive immunoradioassay with radiolabelled antibody. They have been further characterized by immunoblots of GFAP and by immunocytochemistry. Each of the Bigner-Eng antibodies is specific for GFAP and visualizes immunocytochemically astrocytes and astrocytic processes as well as Bergman glia in a wide variety of species. Mixtures of the three Bigner-Eng antibodies provide for a more comprehensive detection of astrocytomas than each antibody alone. Both, anaplastic and reactive astrocytes are stained immunocytochemically. Metastatic tumors and brain tumors of non-astrocytic origin, such as medulloblastomas, meningiomas, choroid plexus papillomas and schwannomas are not stained. There appears to be a positive correlation between fibrillarity of immunocytochemical localization in astrocytomas and their degrees of differentiation, and a negative correlation between the percentage of positive cells and their degree of anaplasticity. In morphologically diagnosed ependymomas and oligodendrogliomas positive reaction appears to reveal presence of malignant astrocytes.
SMI 21 has been tested for immunocytochemical localization of GFAP in astrocytes and Bergmann glia in human, monkey and dog paraffin sections. Astrocytomas from human and dog show a positive reaction with SMI 21. SMI 21 reacts with GFAP and shows no cross reactivity with other intermediate filaments on tissue sections or Western blots of human brain proteins. Rat cytoskeletal proteins do not react with SMI 21.Uses GFAP (SMI 26) Monoclonal Antibody Cocktail This antibody cocktail is effective in immunoblotting, immunocytochemistry and ELISA. Suggested Working Dilution The extent of permissible dilution of SMI 26 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- Immunohistochemistry: 1:1,000
- ELISA: 1:1,000
Tissue Preparation: SMI 26 can be used to detect GFAP in paraffin, vibratome and frozen tissue sections and in cell cultures. In order to facilitate antibody reactivity on paraffin sections, the sections (on glue-coated slides) can be incubated with 0.1% trypsin in 0.05M Tris buffer, pH 7.6, for 20 to 30 minutes at 37°C after paraffin removal. Alternatively, enhanced reaction for GFAP in paraffin sections can be obtained by autoclaving deparaffinized, formalin-fixed sections in distilled water or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min (Evers and Uylings, J Neurosci Methods 72:197, 1997). Post-fixation in methanol or methanol/hydrogen peroxide is necessary for good access of SMI 26 to astrocytes in culture or in thick sections of tissue perfused with 4% paraformaldehyde. Glue-coated slides are prepared by mixing 2.25g of Elmers glue in 15 ml distilled water. Apply one drop of glue solution to a clean glass slide and smear over the slide with another clean slide. Dry overnight in a 56°C oven. Cut paraffin sections, place on glue-covered slide and dry.Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - GFAP (SMI 26) Monoclonal Antibody Cocktail
- xy-0428xy DISC1(disrupted in schizophrenia1)(NT)DISC1 N端抗原
xy-0430xy DISC1(disrupted in schizophrenia1)(CT)DISC1 C端抗原
xy-0421xy DR3/TRAMP (death receptor-3)DR3 死亡受体（抗原）
xy-0714xy E7 protein/HPV 16 (Human papillomavirus type 16)人乳头瘤病毒16型E7蛋白抗原
xy-0165xy EGF-R (Epidemal growth factor receptor)表皮生长因子受体（抗原）
xy-0613xy Eukaryotic aspartyl protease(Rice)天冬氨酸蛋白酶家族蛋白(水稻蛋白的一种)（抗原）
xy-0711xy FAM3B/PANDER(PANcreatic DERived factor)一种新型的胰岛细胞因子(抗原)
xy-0788xy Fabp2 (fatty acid binding protein 2, intestinal)脂肪酸结合蛋白2(抗原)
xy-0500xy ECG2 (sophagus cancer-related gene-2)食道癌相关基因（抗原）
xy-0666xy Fibronectin (FN)纤维连结蛋白(多肽抗原)
xy-0734xy FGF-7/KGF (Keratinocyte growth factor precursor)成纤维细胞生长因子-7/纤维母细胞生长因子 (抗原)
xy-0675xy FGFR2(Fibroblast Growth Factor Receptor 2)成纤维细胞生长因子受体2抗原
xy-0735xy FGF-8/HBGF-8 (Fibroblast growth factor 8)成纤维细胞生长因子-8/纤维母细胞生长因子-8 (抗原)
xy-0400xy GAD-65 (glutamate decarboxylase)谷氨酸脱羧酶-65(C端多肽)
xy-0721xy Galectin-3 半乳糖凝集素-3(抗原)
xy-0701xy GCS (glucosylceramide synthase, GCS)葡萄糖神经酰胺合成酶（抗原）
xy-0639xy GFP绿色荧光蛋白
xy-0725xy ER-alpha雌激素受体alpha
xy-0654xy GHR (growth hormone receptor)生长激素受体(抗原)
xy-0472xy GLUT1(Glucose transporter type 1)葡萄糖转运蛋白1（抗原）
xy-0618xy hypothetical protein (LOC55652)hypothetical protein 抗原
xy-0402xy HCCR-1(BRI3 binding protein)人宫颈癌基因/bri3结合蛋白(抗原)
xy-0343xy pre S1/S2 protein [HBV]乙肝病毒pre S1/乙肝病毒pre S2(抗原)
xy-0728xy HCV-HCBP1/ACY-3(Hepatitis C virus core-binding protein 1)丙型肝炎病毒核心蛋白结合蛋白-1（抗原）
xy-0746xy HHV8/ORF50(Human herpesvirus 8 type P)人类疱疹病毒8抗原
xy-0869xy HHV8/ORF K2/vIL-6(Human herpesvirus 8)人类疱疹病毒8抗原
xy-0737xy HIF-1Alpha缺氧诱导因子1α 多肽
xy-0483xy histone H3-like protein组蛋白H3样蛋白多肽
xy-0620xy histone H3-like protein组蛋白H3样蛋白（C端多肽）
xy-0621xy histone H3-like protein组蛋白H3样蛋白（N端多肽）
xy-0785xy HIV-1(human immunodeficiency virus type 1)艾滋病病毒-1/人类免疫缺陷病毒1型(抗原)
xy-0786xy HIV-1(human immunodeficiency virus type 1)艾滋病病毒-1/人类免疫缺陷病毒1型(抗原)
xy-0887xy Measles virus protein(Plant2 derived measles virus hemagglutinin protein)麻疹病毒红血球凝集素蛋白多肽
xy-0888xy Measles virus protein(Plant2 derived measles virus hemagglutinin protein)麻疹病毒红血球凝集素蛋白多肽
xy-0455xy HSL(hormone-sensitive lipase)荷尔蒙敏感脂肪酸（抗原）
xy-0730xy HSP-27(Heat Shock Protein 27)热休克蛋白-27(抗原)
xy-0696xy HSP-90Alpha(Heat Shock Protein 90Alpha )热休克蛋白90α(抗原)
xy-0135xy HSP-90 Beta (heat sock protein-90 Beta)热休克蛋白-90β （抗原）
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文献和实验相关实验
【翻译】Development trends for monoclonal antibody cancer therapeutics
). However, since 2000, humanized and human mAbs have been entering clinical study at approximately the same rate (4.3 versus 4.5 mAbs per year, respectively). Figure 1 | Categories of monoclonal antibody cancer therapeutics entering clinical study during 1980–1989
Western Blotting with Monoclonal Ant
(1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH7.4). 4.Scrape cells from dish, transfer to a microcentrifuge tube, and boil for an additional 5 minutes. To reduce viscosity, the sample may be sonicated briefly or passed several times through a 26
Western Blotting with Monoclonal Antibodies
several times through a 26-gauge needle. Centrifuge the sample for 5 minutes to pellet insoluble material, then discard pellet. Dilute an aliquot of the cell lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay (SDS
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GFAP (SMI 26) Monoclonal Antibody Cocktail
询价
