- 询价
- R&D
- 美国/中国
- xy-24R
- 2025年07月13日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- GFAP (SMI 24) Monoclonal Antibody保存于运输说明：
- Store at -20°C.
- 详细信息：
-
Description 
Monoclonal antibody against glial fibrillary acidic protein (GFAP) derived from the Bigner-Eng clone MAb2E1 Clone SMI-24 Form Ascites Fluid (contains 0.01M sodium azide) Host Mouse Species Reactivity Human IsoType GFAP (SMI 24) Monoclonal Antibody IgG2b Specificity The Bigner-Eng antibodies have originally been assayed by indirect radioimmunoassay against fixed cell monolayers of a GFAP-positive human glioma cell line and also by competitive radioimmunoassay with radiolabelled GFAP and by competitive immunoradioassay with radiolabelled antibody. They have been further characterized by immunoblots of GFAP and by immunocytochemistry with the peroxidase-antiperoxidase method. While SMI 24 provides for general visualization of GFAP in astrocytes and Bergman glia, it detects a more selected group of astrocytomas than mixtures of SMI 23, 24 and 25. The significance of this selectivity has not yet been explored. Possibilities are different characteristics of individual tumors or cooperativity of antibodies. Uses This antibody is effective in immunoblotting, immunocytochemistry and ELISA. Suggested Working Dilution The extent of permissible dilution of SMI 24 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- Immunohistochemistry: 1:1,000
- GFAP (SMI 24) Monoclonal Antibody ELISA: 1:1,000
Tissue Preparation: SMI 24 can be used to detect GFAP in paraffin, vibratome and frozen tissue sections and in cell cultures. In order to facilitate antibody reactivity on paraffin sections, the sections (on glue-coated slides) can be incubated with 0.1% trypsin in 0.05M Tris buffer, pH 7.6, for 20 to 30 minutes at 37°C after paraffin removal. Glue-coated slides are prepared by mixing 2.25g of Elmers glue in 15 mL distilled water. Apply one drop of glue solution to a clean glass slide and smear over the slide with another clean slide. Dry overnight in a 56°C oven. Cut paraffin sections, place on glue-covered slides and dry. Alternatively, enhanced reaction for GFAP in paraffin sections can be obtained by autoclaving deparaffinized, formalin-fixed sections in distilled water (Shin et al, Lab Invest, 64:693, 1991) or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min (Evers and Uylings, J Neurosci Methods 72:197, 1997). Post-fixation in methanol or methanol/hydrogen peroxide is necessary for access of SMI 24 to astrocytes in culture or in cryostat and vibratome sections of tissue perfused with 4% paraformaldehyde.Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - GFAP (SMI 24) Monoclonal Antibody
- xy-2012xy NIT2 (nitrilase family, member 2)NIT2蛋白抗原
xy-0069xy NKA(Neurokinin A)神经激肽A抗原
xy-2411xy NKG2A/CD159a/KLRC1NK细胞受体2A/自然杀伤细胞活化性受体2A抗原
xy-0938xy NKG2D(natural killer cell group 2D)NK细胞受体/自然杀伤细胞活化性受体
xy-0688xy Nm23(NDP Kinase A,NDPKA)肿瘤转移抑制基因抗原
xy-1066xy Nm23-H1 (nucleoside dIPhosphatase kinase A)肿瘤抑制基因抗原
xy-1315xy Nogo-B/A轴索过度生长抑制因子-B/A抗原
xy-2072xy NOS-2 peptide一氧化氮合成酶-2多肽抗原
xy-1335xy Notch1/MOTC(Neurogenic locus notch homolog protein 1)跨膜受体蛋白Notch-1抗原
xy-1537xy NADPH peptide还原型辅酶Ⅱ抗原
xy-1069xy proCNP/NPPC (pro-natriuretic peptide)促尿钠排泄肽前体C抗原
xy-0937xy NPY2R(Neuropeptide Y Receptor Type 2)神经肽Y受体2（多肽）
xy-1070xy NPY1R (neuropeptide Y1 receptor)神经肽Y1受体抗原
xy-2184xy NQO1(NAD(P)H:quinone oxidoreductase l)醌氧化还原酶抗原
xy-1068xy NR1/NMDAR1(N-Methyl-d-Asprtate receptor 1)谷氨酸受体1（多肽）
xy-2030xy NR1/NMDAR1/GLUR1(N-Methyl-d-Asprtate receptor 1)谷氨酸受体1抗原
xy-2175xy NR1/NMDAR1谷氨酸受体1
xy-1067xy NR2C/NMDAR2C(N-Methyl-d-Asprtate receptor 2C)谷氨酸受体2C（多肽）
xy-1146xy N-ras原癌基因抗原
xy-1074xy Nrf2 peptide核因子2相关因子2抗原
xy-1342xy NRF-1(nuclear respiratory factor-1)核呼吸因子-1抗原
xy-1958xy NTCP(Na+/taurocholate Cotransporting Polypeptide)钠离子/牛磺胆酸共转运蛋白抗原
xy-1375xy Ghrelin 28脑肠肽28
xy-1495xy OCLN(Occluding)咬合蛋白抗原
xy-1111xy Oct-4(Octamer-4)胚胎干细胞关键蛋白抗原
xy-1294xy ODC(Ornithine decarboxylase)鸟氨酸脱羧酶抗原
xy-2086xy ompF(putative outer membrane porin F protein)小肠结肠炎耶尔森氏菌膜通道蛋白抗原
xy-2173xy OLFM1 peptide嗅球蛋白1多肽抗原
xy-1181xy ORX-A(orexin-A)增食欲素-A/欲激素A抗原
xy-1314xy OTR(oxytocin receptor)缩宫素受体
xy-1095xy OXR (Orexin receptor)食欲素受体(多肽)
xy-1483xy P2P-R/RBBP6(proliferation potential protein related)增殖潜能相关蛋白抗原
xy-0534xy P19ARFp19ARF抑癌基因抗原
xy-2210xy phospho-p38 ERK/MAPK14 (pThr180/pTyr182) peptide磷酸化丝裂原活化蛋白激酶p38抗原
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文献和实验相关实验
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Positive Clones (1) The day prior to any expansion,obtain feeder cells.For this first expansion from the 200 µl cultures in 96-well plates to 500 µl cultures in 24-well plates,add 200 µl of feeder cells/well in DMEM/20% FCSHT to the 24-well plates the day
Monoclonal Antibody Production Protocol
/ml). Add Aminopterin (2ml to 200 ml of medium). (Some workers will leave the cells at this point for 24 hours before adding Aminopterin. We add the drug immediately.) Seed the cells in 96 well microtiter dishes, 250 ul per well, 8 plates per fusion
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GFAP (SMI 24) Monoclonal Antibody
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