- 询价
- R&D
- 美国/中国
- xy-25R
- 2025年06月12日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- GFAP (SMI 25) Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal antibody against glial fibrillary acidic protein (GFAP) derived from the Bigner-Eng clone MAb 4A11 Clone SMI-25 Form Ascites Fluid Host Mouse Species Reactivity GFAP (SMI 25) Monoclonal Antibody Human IsoType IgG2b Specificity The Bigner-Eng antibodies have originally been assayed by indirect radioimmunoassay against fixed cell monolayers of a GFAP-positive human glioma cell line and also by competitive radioimmunoassay with radiolabelled GFAP and by competitive immunoradioassay with radiolabelled antibody. They have been further characterized by immunoblots of GFAP and by immunocytochemistry with the peroxidase-antiperoxidase method. While SMI 25 provides for general visualization of GFAP in astrocytes and Bergman glia, it detects a more selected group of astrocytomas than mixtures of SMI 23, 24 and 25. The significance of this selectivity has not yet been explored. Possibilities are different characteristics of individual tumors or cooperativity of antibodies. Uses This antibody is effective in immunoblotting, immunocytochemistry and ELISA. Suggested Working Dilution The extent of permissible dilution of SMI 25 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- GFAP (SMI 25) Monoclonal Antibody Immunohistochemistry: 1:1,000
- ELISA: 1:1,000
Tissue Preparation: SMI 25 can be used to detect GFAP in paraffin, vibratome and frozen tissue sections and in cell cultures. In order to facilitate antibody reactivity on paraffin sections, the sections (on glue-coated slides) can be incubated with 0.1% trypsin in 0.05M Tris buffer, pH 7.6, for 20 to 30 minutes at 37oC after paraffin removal. Glue-coated slides are prepared by mixing 2.25g of Elmers glue in 15 ml distilled water. Apply one drop of glue solution to a clean glass slide and smear over the slide with another clean slide. Dry overnight in a 56oC oven. Cut paraffin sections, place on glue-covered slides and dry. Alternatively, enhanced reaction for GFAP in paraffin sections can be obtained by autoclaving deparaffinized, formalin-fixed sections in distilled water as described by Shin et al, Lab Invest, 64:693,1991, or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min (Evers and Uylings, J Neurosci Methods 72:197, 1997). Post-fixation in methanol or methanol/hydrogen peroxide is necessary for good access of SMI 25 to astrocytes in culture or in cryostat and vibratome sections of tissue perfused with 4% paraformaldehyde.Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - GFAP (SMI 25) Monoclonal Antibody
- xy-2446xy phospho-eIF4E(pSer209)(phospho-Eukaryotic translation initiation factor 4E)磷酸化eIF-4E（Ser209）抗原
xy-1398xy ELK1细胞转录因子ELK1抗原
xy-1756xy Elastin弹性蛋白抗原
xy-2651xy ELAVL1/HuR peptideELAVL1/HuR多肽抗原
xy-0558xy Emp1 (epithelial membrane protein 1)表皮膜蛋白1抗原
xy-1331xy sFRP-4(Secreted frizzled-related protein 4; Frizzled protein, human endometrium)抗子宫内膜抗原
xy-0547xy Endostatin内皮抑素/内皮他丁抗原
xy-0658xy E.coli O6 (Escherichia coli O6)E.coli O6大肠杆菌菌体蛋白
xy-2100xy EPEC/E.coli(enteropathogenic E．coli，EPEC)普通大肠埃希菌菌体蛋白（非致病性）
xy-1763xy EphA1 R(Ephrin A1 Receptor)EphA1-R受体抗原
xy-1424xy EPOR peptide红细胞生成素受体抗原
xy-1522xy phospho-ERK1/MAPK-1/2(pThr183 + pTyr185)磷酸化丝裂原活化蛋白激酶1/2抗原
xy-0996xy ERK(Extracellular signal-regulated kinase)丝裂原活化蛋白激酶抗原
xy-1020xy MAPK1/ERK3 (Mitogen-activated protein kinase 3; P44MAPK)丝裂原活化蛋白激酶3抗原
xy-1319xy MAPK4(Mitogen-activated protein kinase 4)丝裂原活化蛋白激酶4抗原
xy-0894xy ER- Alpha(phospho-Ser167)peptide人磷酸化雌激素受体α多肽抗原
xy-1643xy ER- Alpha(phospho-Tyr537)peptide磷酸化雌激素受体α抗原
xy-0253xy ER- Alpha /ER alpha peptide雌激素受体α抗原
xy-2098xy ER- Alpha/ER alpha peptide雌激素受体α抗原
xy-0255xy ER- Beta (Estrogen receptor-beta) peptide雌激素受体β抗原
xy-1285xy ets1/E26 (E-twenty six 1)Ets转录因子家族ets1抗原
xy-0983xy EV71(Human enterovirus 71)VP1(CT)肠道病毒71型/手足口病病毒抗原(C端)
xy-2221xy ATEXPA10 peptide植物扩张蛋白ATEXPA10（拟南芥）抗原
xy-1343xy Ezrin/Radixin埃兹蛋白（多肽）
xy-0949xy F1 operon positive regulatory protein(NT)鼠疫耶尔森氏菌F1荚膜蛋白(N端多肽）
xy-0950xy F1 operon positive regulatory protein(CT)鼠疫耶尔森氏菌F1荚膜蛋白(C端多肽）
xy-2135xy FABP(brain) (Fatty acid-binding protein brain)脑型脂肪酸结合蛋白抗原
xy-0511xy FADD (Fas-associated protein with death domain)Fas死亡结构域相关蛋白抗原
xy-1340xy FAK(Focaladhesin kinase)粘着斑激酶抗原
xy-1639xy phospho-FAK(pTyr577)磷酸化粘着斑激酶抗原
xy-1642xy phospho-FAK(pSer732)(phospho-Focal adhesion kinase)磷酸化粘着斑激酶抗原
xy-1765xy FAST kinase(Fas-activated serine/threonine kinase)Fas活化的丝/苏氨酸激酶抗原
xy-1157xy FBN1 (fibrillin 1)原纤维蛋白1抗原
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GFAP (SMI 25) Monoclonal Antibody
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